Thermoresponsive polymer brush surfaces with hydrophobic groups for all-aqueous chromatography

Kenichi Nagase, Mio Kumazaki, Hideko Kanazawa, Jun Kobayashi, Akihiko Kikuci, Yoshikatsu Akiyama, Masahiko Annaka, Teruo Okano

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)


For developing thermoresponsive chromatographic matrices with a strong hydrophobicity, poly(N-isopropylacrylamide-co-n-butyl methacrylate) (poly(IPAAm-co-BMA)) brush grafted silica beads were prepared through a surface-initiated atom transfer radical polymerization (ATRP) with a CuCl/CuCl2/Me6TREN catalytic system in 2-propanol at 25 °C for 16 h. The prepared beads were characterized by chromatographic analysis. Chromatograms of the benzoic-acid family and phenol as model analytes were obtained with high-resolution peaks because of their strong hydrophobic interactions to the densely grafted hydrophobized copolymers on the beads. Retention times of the analytes increased with the increase in BMA composition ratio. Dehydration of grafted copolymer with large BMA composition was performed at low temperature. These results indicated that the copolymer-brush-grafted surface prepared by ATRP was an effective tool for separating hydrophilic analytes at low temperature through modulating the strong hydrophobic interaction.

Original languageEnglish
Pages (from-to)1247-1253
Number of pages7
JournalACS Applied Materials and Interfaces
Issue number4
Publication statusPublished - 2010 Apr 28


  • Chromatography
  • Hydrophobic interaction
  • Polymer brush
  • Separation
  • Thermoresponsive surface

ASJC Scopus subject areas

  • General Materials Science


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