Thermoresponsive Poly(N-isopropylacrylamide)-Based Block Copolymer Coating for Optimizing Cell Sheet Fabrication

Masamichi Nakayama, Naoko Yamada, Yoshikazu Kumashiro, Hideko Kanazawa, Masayuki Yamato, Teruo Okano

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)


Thermoresponsive surfaces are prepared via a spin-coating method with a block copolymer consisting of poly(N-isopropylacrylamide) (PIPAAm) and poly(butyl methacrylate) (PBMA) on polystyrene surfaces. The PBMA block suppresses the removal of deposited PIPAAm-based polymers from the surface. The polymer coating affects the temperature-dependent cellular behavior of the surfaces with respect to protein adsorption. By adjusting layer thicknesses, PBMA-b-PIPAAm-coated surfaces are optimized to regulate the adhesion/detachment of cells by temperature changes. Thus, thermoresponsive polymer-coated surfaces are able to harvest contiguous cell sheets with their basal extracellular matrix proteins.

Original languageEnglish
Pages (from-to)751-760
Number of pages10
JournalMacromolecular Bioscience
Issue number6
Publication statusPublished - 2012 Jun


  • Adhesion
  • Block copolymers
  • Cells
  • Poly(N-isopropylacrylamide)
  • Spin coating

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry


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