TY - GEN
T1 - Three dimensional spheroids formation platform using micro-rotation flow
AU - Ota, Hiroki
AU - Deguchi, Koji
AU - Kita, Shiny
AU - Yamamoto, Ryosuke
AU - Miki, Norihisa
PY - 2008
Y1 - 2008
N2 - Hepatocytes are responsible for metabolic and detoxification processes in the liver. During the tissue formation from isolated cells, they increase the cell density and acquire their liver-specific functions, new protein expression and cell signaling. Hence, tissue-based in vitro studies are strongly demanded. We develop a MEMS platform which forms 3D spheroids by microfluodic handing of cells. The microfludic platform is composed of PDMS chamber fabricated by microlithography and a circulation system. We simulated the flow in the chamber by FLUENT and found that micro-rotation flow was generated when the chamber height was four times greater than the channel height. The rotation flow was experimentally observed using fluorescent microbeads with the newly designed micro-chamber. The proposed MEMS-based 3D-spheroids formation platform is readily to create a technological innovation in biology as a bridge between cell researches and tissue researches.
AB - Hepatocytes are responsible for metabolic and detoxification processes in the liver. During the tissue formation from isolated cells, they increase the cell density and acquire their liver-specific functions, new protein expression and cell signaling. Hence, tissue-based in vitro studies are strongly demanded. We develop a MEMS platform which forms 3D spheroids by microfluodic handing of cells. The microfludic platform is composed of PDMS chamber fabricated by microlithography and a circulation system. We simulated the flow in the chamber by FLUENT and found that micro-rotation flow was generated when the chamber height was four times greater than the channel height. The rotation flow was experimentally observed using fluorescent microbeads with the newly designed micro-chamber. The proposed MEMS-based 3D-spheroids formation platform is readily to create a technological innovation in biology as a bridge between cell researches and tissue researches.
UR - http://www.scopus.com/inward/record.url?scp=62449226376&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=62449226376&partnerID=8YFLogxK
U2 - 10.1109/MHS.2008.4752450
DO - 10.1109/MHS.2008.4752450
M3 - Conference contribution
AN - SCOPUS:62449226376
SN - 9781424429196
T3 - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008
SP - 202
EP - 207
BT - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"
T2 - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation"
Y2 - 6 November 2008 through 9 November 2008
ER -