Tissue engineering of skeletal muscle in collagen gel

Kazuo Kishi, Hiroko Satoh, Takara Tanaka, Yoshiaki Kubota, Taroh Koyama, Nobuaki Imanishi, Hideo Nakajima, Tatsuo Nakajima

Research output: Contribution to journalArticlepeer-review


In order to construct functional skeletal muscle in vitro for tissue engineering, cell alignment followed by the fusion of skeletal muscle cells, and the construction of basement membrane are needed. Of the various means used to align skeletal muscle cells, the mechanical load method is the most popular. In the present paper, we describe the history of attempts to align skeletal muscle cells in vitro and the construction of basement membrane. We then describe the challenges we faced to achieve skeletal muscle tissue engineering in type I collagen gel by co-culturing various types of cells including skeletal muscle cells, fibroblasts, endothelial cells, and fetal rat anterior horn cells.

Original languageEnglish
Pages (from-to)1001-1005
Number of pages5
JournalJapanese Journal of Plastic and Reconstructive Surgery
Issue number9
Publication statusPublished - 2004 Sept

ASJC Scopus subject areas

  • Surgery


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