TY - JOUR
T1 - Tissue-specific expression and mapping of the Cox7ah gene in mouse
AU - Jaradat, Saied A.
AU - Ko, Minoru S.H.
AU - Grossman, Lawrence I.
N1 - Funding Information:
This research was supported by NIH GM48517 and by a grant from the Muscular Dystrophy Foundation to L.I.G. and also by NIH Grant HD32243 to M.S.H.K. We thank Timothy R. Schmidt for his help in computer analysis, Orlando J. Miller for helpful discussions, and Lucy Rowe for help with the map data analyses. S.A.J. was partially supported by a fellowship from Jordan University of Science and Technology. We also thank two anonymous reviewers for helpful suggestions, including an improved 3′ alignment in Fig. 2.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1998/5/1
Y1 - 1998/5/1
N2 - We have isolated and examined the gene for the heart isoform of cytochrome c oxidase subunit VIIa (COX VIIa-H) in mouse, an isoform gene previously thought to be lacking in rodents. Interspecies amino acid comparisons indicate that mouse COX VIIa-H protein displays 82.5 and 70.9% identity with the bovine and human heart isoforms of COX VIIa, but only 53.7% identity with the paralogous mouse liver isoform (COX VIIa-L). Expression in adult mouse tissues is limited to heart and skeletal muscle, as found in other species. In the early mouse embryo, Cox7al was the exclusive isoform expressed and Cox7ah mRNA was not detectable until day 17 postcoitum. That the mouse Cox7ah gene characterized in this study is orthologous to the human COX7AH gene was also suggested by its mapping to mouse chromosome 7, to a conserved region syntenic with the human chromosome location of COX7AH, 19q13.1. As a result, all three COX heart isoform genes in mouse group to chromosome 7. Interestingly, mapping of the mouse Cox7al to chromosome 9 suggests a new syntenic region between the mouse and the human genomes.
AB - We have isolated and examined the gene for the heart isoform of cytochrome c oxidase subunit VIIa (COX VIIa-H) in mouse, an isoform gene previously thought to be lacking in rodents. Interspecies amino acid comparisons indicate that mouse COX VIIa-H protein displays 82.5 and 70.9% identity with the bovine and human heart isoforms of COX VIIa, but only 53.7% identity with the paralogous mouse liver isoform (COX VIIa-L). Expression in adult mouse tissues is limited to heart and skeletal muscle, as found in other species. In the early mouse embryo, Cox7al was the exclusive isoform expressed and Cox7ah mRNA was not detectable until day 17 postcoitum. That the mouse Cox7ah gene characterized in this study is orthologous to the human COX7AH gene was also suggested by its mapping to mouse chromosome 7, to a conserved region syntenic with the human chromosome location of COX7AH, 19q13.1. As a result, all three COX heart isoform genes in mouse group to chromosome 7. Interestingly, mapping of the mouse Cox7al to chromosome 9 suggests a new syntenic region between the mouse and the human genomes.
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U2 - 10.1006/geno.1998.5279
DO - 10.1006/geno.1998.5279
M3 - Article
C2 - 9615220
AN - SCOPUS:0031863048
SN - 0888-7543
VL - 49
SP - 363
EP - 370
JO - Genomics
JF - Genomics
IS - 3
ER -