Toll-like receptors, NOD1, and NOD2 in oral epithelial cells

Y. Sugawara, A. Uehara, Y. Fujimoto, S. Kusumoto, K. Fukase, K. Shibata, S. Sugawara, T. Sasano, H. Takada

Research output: Contribution to journalArticlepeer-review

136 Citations (Scopus)


Oral epithelium might be the first barrier against oral bacteria in periodontal tissue. We hypothesized that oral epithelium is endowed with innate immune receptors for bacterial components, which play roles in host defense against bacterial infection without being accompanied by excessive inflammatory responses. We found clear expression of Toll-like receptor (TLR)4 as well as TLR2, and strong expression of NOD1 and NOD2 in normal oral epithelial tissues by immunohistochemical analysis. We also showed that primary oral epithelial cells in culture expressed these molecules using PCR, flow cytometry, and immunostaining. In inflamed oral epithelium, cell-surface localizations of TLR2 and TLR4 were more clearly observed than in healthy tissue. Upon stimulation with synthetic ligands for these receptors, the expression of β-defensin 2 was markedly up-regulated. These findings indicate that these molecules in oral epithelial cells are functional receptors that induce antibacterial responses.

Original languageEnglish
Pages (from-to)524-529
Number of pages6
JournalJournal of Dental Research
Issue number6
Publication statusPublished - 2006 Jun
Externally publishedYes


  • Epithelium
  • Inflammation
  • NOD
  • TLR
  • β-defensin 2

ASJC Scopus subject areas

  • Dentistry(all)


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