Abstract
The advent of genome synthesis in the 21st century opened up various approaches on cloning whole bacterial genomes. In particular, relevant technologies starting from chemically synthesized oligomers should enable genome reorganization not limited to existing genomes but expanded to any genomes with novel sequences designed even from scratch. Those cutting-edge technologies sooner or later will replace template-dependent gene cloning technologies, albeit technical difficulties regarding efficiency and cost preclude general use at present. Meanwhile, rationally designed genomes require thorough knowledge of existing genomes in retrospect. Certain unnoticed rules underlying functional genomes might be found through various kinds and levels of studies.This chapter recaps the presently available tools and relevant ideas towards production of giant DNA molecules equivalent to or potentially larger-sized bacterial genomes, particularly focusing on our host, Bacillus subtilis 168. This chapter also describes the future perspectives of deep understanding on cellular systems innovated by and viewed from the genome synthesis technology. © 2013
| Original language | English |
|---|---|
| Title of host publication | Synthetic Biology |
| Publisher | Elsevier Inc. |
| Pages | 225-242 |
| Number of pages | 18 |
| ISBN (Print) | 9780123944306 |
| DOIs | |
| Publication status | Published - 2013 |
Keywords
- Bacillus subtilis
- DNA replication
- Gene expression
- Genome vector
- Homologous recombination
- Host and guest genomes
- Largest genomes
- Smallest genomes
- Transformation
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)