Transplantation of human limbal epithelium cultivated on amniotic membrane for the treatment of severe ocular surface disorders

Jun Shimazaki, Masayo Aiba, Eiki Goto, Naoko Kato, Shigeto Shimmura, Kazuo Tsubota

Research output: Contribution to journalArticlepeer-review

287 Citations (Scopus)


Purpose: To study the short-term clinical results of transplanting of cultivated corneal/limbal epithelial cells on human amniotic membrane (AM) for limbal deficiency. Design: Noncomparative, retrospective interventional case series. Participants: Thirteen eyes of 13 patients with severe limbal deficiency (Stevens-Johnson syndrome in eight eyes, ocular cicatricial pemphigoid in three eyes, and chemical burns in two eyes) were treated at the department of Ophthalmology, Tokyo Dental College, Japan. Intervention: Cultivated allo-limbal epithelium was transplanted onto the ocular surface of patients with severe limbal deficiency. Main Outcome Measures: Ocular surface reconstruction with corneal epithelialization, changes in visual acuity, and postoperative complications were studied. Histologic examinations were also performed on cultivated epithelium. Results: Cultivated corneal epithelium on AM formed two to three layers with the formation of basement membrane-like structures. After the surgery, the epithelium regenerated and covered the ocular surface in eight eyes (61.5%). However, three of the eight eyes developed partial conjunctival invasion, and two eyes later developed epithelial defects. At last examination, corneal epithelialization was achieved in six eyes (46.2%). Five eyes had conjunctivalization, one eye had dermal epithelialization, and one eye was not epithelialized. Complications were corneal perforation in four eyes and infectious keratitis in two eyes. Conclusions: This study demonstrates that the success rate for transplanting cultivated allo-limbal epithelium on the AM is not different from the conventional limbal and AM transplantation for the treatment of severe limbal stem cell dysfunction.

Original languageEnglish
Pages (from-to)1285-1290
Number of pages6
Issue number7
Publication statusPublished - 2002
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology


Dive into the research topics of 'Transplantation of human limbal epithelium cultivated on amniotic membrane for the treatment of severe ocular surface disorders'. Together they form a unique fingerprint.

Cite this