Tumor suppressor candidate 5 (TUSC5) is expressed in brown adipocytes

Hisashi Koide; Takahisa Shibata; Nobuko Yamada; Toshiyuki Asaki; Toshitaka Nagao; Tomohiko Yoshida; Yoshihiko Noguchi; Tomoaki Tanaka; Yasushi Saito; Ichiro Tatsuno

doi:10.1016/j.bbrc.2007.06.034

Tumor suppressor candidate 5 (TUSC5) is expressed in brown adipocytes

Hisashi Koide, Takahisa Shibata, Nobuko Yamada, Toshiyuki Asaki, Toshitaka Nagao, Tomohiko Yoshida, Yoshihiko Noguchi, Tomoaki Tanaka, Yasushi Saito, Ichiro Tatsuno

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Rat brain endothelial cell derived gene-1 (BEC-1) had considerable homology with tumor suppressor candidate 5 (TUSC5). TUSC5 was expressed abundantly, and its mRNA was inhibited by cold exposure in rat brown adipose tissue (BAT). In the present study, we investigated its regulatory mechanism using primary cultured rat brown preadipocytes (RBPA) and Zucker lean rats (ZL). We found that: (1) TUSC5 mRNA began to increase in a manner similar to C/EBP-α, PPAR-γ, and adiponectin during differentiation in RBPA; (2) neither β3-adrenoceptor agonist BRL 37344 nor dexamethasone affected TUSC5 mRNA in RBPA; (3) propranolol did not block the decrease of TUSC5 mRNA by cold exposure in ZL; (4) BRL 37344 did not influence TUSC5 mRNA in ZL; and (5) dexamethasone inhibited TUSC5 mRNA in a dose-dependent manner similar to UCP-1 in ZL. These data suggested that TUSC5 is involved in the differentiation, and its expression is regulated independently of the β-adrenergic pathway in BAT.

Original language	English
Pages (from-to)	139-145
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	360
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2007.06.034
Publication status	Published - 2007 Aug 17
Externally published	Yes

Keywords

Adipocyte
BEC-1
Brown adipose tissue
Obese
TUSC5

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2007.06.034

Cite this

@article{dfcdccc370864877b6be8ef0cdc1a746,

title = "Tumor suppressor candidate 5 (TUSC5) is expressed in brown adipocytes",

abstract = "Rat brain endothelial cell derived gene-1 (BEC-1) had considerable homology with tumor suppressor candidate 5 (TUSC5). TUSC5 was expressed abundantly, and its mRNA was inhibited by cold exposure in rat brown adipose tissue (BAT). In the present study, we investigated its regulatory mechanism using primary cultured rat brown preadipocytes (RBPA) and Zucker lean rats (ZL). We found that: (1) TUSC5 mRNA began to increase in a manner similar to C/EBP-α, PPAR-γ, and adiponectin during differentiation in RBPA; (2) neither β3-adrenoceptor agonist BRL 37344 nor dexamethasone affected TUSC5 mRNA in RBPA; (3) propranolol did not block the decrease of TUSC5 mRNA by cold exposure in ZL; (4) BRL 37344 did not influence TUSC5 mRNA in ZL; and (5) dexamethasone inhibited TUSC5 mRNA in a dose-dependent manner similar to UCP-1 in ZL. These data suggested that TUSC5 is involved in the differentiation, and its expression is regulated independently of the β-adrenergic pathway in BAT.",

keywords = "Adipocyte, BEC-1, Brown adipose tissue, Obese, TUSC5",

author = "Hisashi Koide and Takahisa Shibata and Nobuko Yamada and Toshiyuki Asaki and Toshitaka Nagao and Tomohiko Yoshida and Yoshihiko Noguchi and Tomoaki Tanaka and Yasushi Saito and Ichiro Tatsuno",

year = "2007",

month = aug,

day = "17",

doi = "10.1016/j.bbrc.2007.06.034",

language = "English",

volume = "360",

pages = "139--145",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Tumor suppressor candidate 5 (TUSC5) is expressed in brown adipocytes

AU - Koide, Hisashi

AU - Shibata, Takahisa

AU - Yamada, Nobuko

AU - Asaki, Toshiyuki

AU - Nagao, Toshitaka

AU - Yoshida, Tomohiko

AU - Noguchi, Yoshihiko

AU - Tanaka, Tomoaki

AU - Saito, Yasushi

AU - Tatsuno, Ichiro

PY - 2007/8/17

Y1 - 2007/8/17

N2 - Rat brain endothelial cell derived gene-1 (BEC-1) had considerable homology with tumor suppressor candidate 5 (TUSC5). TUSC5 was expressed abundantly, and its mRNA was inhibited by cold exposure in rat brown adipose tissue (BAT). In the present study, we investigated its regulatory mechanism using primary cultured rat brown preadipocytes (RBPA) and Zucker lean rats (ZL). We found that: (1) TUSC5 mRNA began to increase in a manner similar to C/EBP-α, PPAR-γ, and adiponectin during differentiation in RBPA; (2) neither β3-adrenoceptor agonist BRL 37344 nor dexamethasone affected TUSC5 mRNA in RBPA; (3) propranolol did not block the decrease of TUSC5 mRNA by cold exposure in ZL; (4) BRL 37344 did not influence TUSC5 mRNA in ZL; and (5) dexamethasone inhibited TUSC5 mRNA in a dose-dependent manner similar to UCP-1 in ZL. These data suggested that TUSC5 is involved in the differentiation, and its expression is regulated independently of the β-adrenergic pathway in BAT.

AB - Rat brain endothelial cell derived gene-1 (BEC-1) had considerable homology with tumor suppressor candidate 5 (TUSC5). TUSC5 was expressed abundantly, and its mRNA was inhibited by cold exposure in rat brown adipose tissue (BAT). In the present study, we investigated its regulatory mechanism using primary cultured rat brown preadipocytes (RBPA) and Zucker lean rats (ZL). We found that: (1) TUSC5 mRNA began to increase in a manner similar to C/EBP-α, PPAR-γ, and adiponectin during differentiation in RBPA; (2) neither β3-adrenoceptor agonist BRL 37344 nor dexamethasone affected TUSC5 mRNA in RBPA; (3) propranolol did not block the decrease of TUSC5 mRNA by cold exposure in ZL; (4) BRL 37344 did not influence TUSC5 mRNA in ZL; and (5) dexamethasone inhibited TUSC5 mRNA in a dose-dependent manner similar to UCP-1 in ZL. These data suggested that TUSC5 is involved in the differentiation, and its expression is regulated independently of the β-adrenergic pathway in BAT.

KW - Adipocyte

KW - BEC-1

KW - Brown adipose tissue

KW - Obese

KW - TUSC5

UR - http://www.scopus.com/inward/record.url?scp=34250849055&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250849055&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2007.06.034

DO - 10.1016/j.bbrc.2007.06.034

M3 - Article

C2 - 17592729

AN - SCOPUS:34250849055

SN - 0006-291X

VL - 360

SP - 139

EP - 145

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Tumor suppressor candidate 5 (TUSC5) is expressed in brown adipocytes

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this