Two new lignans and melanogenesis inhibitors from Schisandra nigra

Yuji Narukawa, Chihiro Komatsu, Rina Yamauchi, Sakiko Shibayama, Mayuko Hachisuka, Fumiyuki Kiuchi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


An acetone extract from the stems of Schisandra nigra Max. (Schisandraceae) exhibited significant inhibition of 3-isobutyl-1-methylxanthine (IBMX)-stimulated melanogenesis in murine B16 melanoma F10 cells. Fractionation and purification of the extract led to the isolation of two new tetrahydrofuran-type lignans, (+)-5-methoxyzuonin A (2) and kadlongirin C (3), along with eight known compounds (1, 4–10). The structures of the new compounds were determined by spectroscopic analyses. Of the isolated compounds (1, 3 –10), (+)-zuonin A (1) showed remarkable inhibition of melanogenesis at concentrations without cytotoxicity in B16 melanoma F10 cells. (+)-Zuonin A (1) did not inhibit tyrosinase; however, Western blot analysis revealed that it decreased protein levels of tyrosinase and tyrosinase-related proteins (TRP)-1 and TRP-2 without changing phosphorylation level of cAMP response element-binding protein.

Original languageEnglish
Pages (from-to)460-466
Number of pages7
JournalJournal of Natural Medicines
Issue number3
Publication statusPublished - 2016 Jul 1


  • Melanogenesis inhibitors
  • Schisandra nigra
  • Tetrahydrofuran-type lignans

ASJC Scopus subject areas

  • Molecular Medicine


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