Ultraprecision cutting of single-crystal calcium fluoride for fabricating micro flow cell for protein structure analysis

Ryo Komiya; Tetsunari Kimura; Minoru Kubo; Jiwang Yan

Ultraprecision cutting of single-crystal calcium fluoride for fabricating micro flow cell for protein structure analysis

Ryo Komiya, Tetsunari Kimura, Minoru Kubo, Jiwang Yan

Department of Mechanical Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

In infrared spectroscopic analysis of the structures of protein crystals, a micro-flow cell made of single crystal calcium fluoride (CaF₂) is needed. In this research, fly cutting of micro grooves on a CaF₂ substrate was conducted using a single-crystal diamond tool. The effects of cutting direction, up/down cut, and tool rake angle on ductile-brittle transition of cutting behavior were investigated. Based on the experimental results and analysis, optimal cutting conditions were selected which enabled ductile machining of CaF₂. As a result, a 10 μm deep micro-flow cell was successfully fabricated with surface roughness of 2.4 nmRa. The availability of the flow cell was confirmed by visible absorption spectroscopy of protein.

Original language	English
Publication status	Published - 2015 Oct 18
Event	8th International Conference on Leading Edge Manufacturing in 21st Century, LEM 2015 - Kyoto, Japan Duration: 2015 Oct 18 → 2015 Oct 22

Other

Other	8th International Conference on Leading Edge Manufacturing in 21st Century, LEM 2015
Country/Territory	Japan
City	Kyoto
Period	15/10/18 → 15/10/22

Keywords

Calcium fluoride
Ductile machining
Flow cell
Micro machining
Ultraprecision cutting

ASJC Scopus subject areas

Industrial and Manufacturing Engineering

Cite this

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title = "Ultraprecision cutting of single-crystal calcium fluoride for fabricating micro flow cell for protein structure analysis",

abstract = "In infrared spectroscopic analysis of the structures of protein crystals, a micro-flow cell made of single crystal calcium fluoride (CaF2) is needed. In this research, fly cutting of micro grooves on a CaF2 substrate was conducted using a single-crystal diamond tool. The effects of cutting direction, up/down cut, and tool rake angle on ductile-brittle transition of cutting behavior were investigated. Based on the experimental results and analysis, optimal cutting conditions were selected which enabled ductile machining of CaF2. As a result, a 10 μm deep micro-flow cell was successfully fabricated with surface roughness of 2.4 nmRa. The availability of the flow cell was confirmed by visible absorption spectroscopy of protein.",

keywords = "Calcium fluoride, Ductile machining, Flow cell, Micro machining, Ultraprecision cutting",

author = "Ryo Komiya and Tetsunari Kimura and Minoru Kubo and Jiwang Yan",

year = "2015",

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day = "18",

language = "English",

note = "8th International Conference on Leading Edge Manufacturing in 21st Century, LEM 2015 ; Conference date: 18-10-2015 Through 22-10-2015",

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TY - CONF

T1 - Ultraprecision cutting of single-crystal calcium fluoride for fabricating micro flow cell for protein structure analysis

AU - Komiya, Ryo

AU - Kimura, Tetsunari

AU - Kubo, Minoru

AU - Yan, Jiwang

PY - 2015/10/18

Y1 - 2015/10/18

N2 - In infrared spectroscopic analysis of the structures of protein crystals, a micro-flow cell made of single crystal calcium fluoride (CaF2) is needed. In this research, fly cutting of micro grooves on a CaF2 substrate was conducted using a single-crystal diamond tool. The effects of cutting direction, up/down cut, and tool rake angle on ductile-brittle transition of cutting behavior were investigated. Based on the experimental results and analysis, optimal cutting conditions were selected which enabled ductile machining of CaF2. As a result, a 10 μm deep micro-flow cell was successfully fabricated with surface roughness of 2.4 nmRa. The availability of the flow cell was confirmed by visible absorption spectroscopy of protein.

AB - In infrared spectroscopic analysis of the structures of protein crystals, a micro-flow cell made of single crystal calcium fluoride (CaF2) is needed. In this research, fly cutting of micro grooves on a CaF2 substrate was conducted using a single-crystal diamond tool. The effects of cutting direction, up/down cut, and tool rake angle on ductile-brittle transition of cutting behavior were investigated. Based on the experimental results and analysis, optimal cutting conditions were selected which enabled ductile machining of CaF2. As a result, a 10 μm deep micro-flow cell was successfully fabricated with surface roughness of 2.4 nmRa. The availability of the flow cell was confirmed by visible absorption spectroscopy of protein.

KW - Calcium fluoride

KW - Ductile machining

KW - Flow cell

KW - Micro machining

KW - Ultraprecision cutting

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M3 - Paper

AN - SCOPUS:84974628493

T2 - 8th International Conference on Leading Edge Manufacturing in 21st Century, LEM 2015

Y2 - 18 October 2015 through 22 October 2015

ER -

Ultraprecision cutting of single-crystal calcium fluoride for fabricating micro flow cell for protein structure analysis

Abstract

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Keywords

ASJC Scopus subject areas

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