Ultrasound-induced DNA damage and signal transductions indicated by gammaH2AX

Yukihiro Furusawa, Yoshisada Fujiwara, Qing Li Zhao, Mariame Ali Hassan, Ryohei Ogawa, Yoshiaki Tabuchi, Ichiro Takasaki, Akihisa Takahashi, Takeo Ohnishi, Takashi Kondo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)


Ultrasound (US) has been shown to induce cancer cell death via different forms including apoptosis. Here, we report the potential of low-intensity pulsed US (LIPUS) to induce genomic DNA damage and subsequent DNA damage response. Using the ionizing radiation-induced DNA doublestrand breaks (DSBs) as the positive control, we were able to observe the induction of DSBs (as neutral comet tails) and the subsequent formation of gammaH2AX-positive foci (by immunofluorescence detection) in human leukemia cells following exposure to LIPUS. The LIPUS-induced DNA damage arose most likely from the mechanical, but not sonochemical, effect of cavitation, based on our observation that the suppression of inertial cavitation abrogated the gammH2AX foci formation, whereas scavenging of free radical formation (e.g., hydroxyl radical) had no protective effect on it. Treatment with the specific kinase inhibitor of ATM or DNA-PKcs, which can phosphorylate H2AX Ser139, revealed that US-induced gammaH2AX was inhibited more effectively by the DNA-PK inhibitor than ATM kinase inhibitor. Notably, these inhibitor effects were opposite to those with radiation-induced gammH2AX. In conclusion, we report, for the first time that US can induce DNA damage and the DNA damage response as indicated by gammaH2AX was triggered by the cavitational mechanical effects. Thus, it is expected that the data shown here may provide a better understanding of the cellular responses to US.

Original languageEnglish
Title of host publication10th International Symposium on Therapeutic Ultrasound, ISTU 2010
Number of pages4
Publication statusPublished - 2011
Externally publishedYes
Event10th International Symposium on Therapeutic Ultrasound, ISTU 2010 - Tokyo, Japan
Duration: 2010 Jun 92010 Jun 12

Publication series

NameAIP Conference Proceedings
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616


Other10th International Symposium on Therapeutic Ultrasound, ISTU 2010


  • Cavitation
  • DNA damage
  • GammaH2AX
  • Ultrasound

ASJC Scopus subject areas

  • General Physics and Astronomy


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