Ultrasound-Induced New Cellular Mechanism Involved in Drug Resistance

Mariame A. Hassan; Yukihiro Furusawa; Masami Minemura; Natalya Rapoport; Toshiro Sugiyama; Takashi Kondo

doi:10.1371/journal.pone.0048291

Ultrasound-Induced New Cellular Mechanism Involved in Drug Resistance

Mariame A. Hassan, Yukihiro Furusawa, Masami Minemura, Natalya Rapoport, Toshiro Sugiyama, Takashi Kondo

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

The acoustic effects in a biological milieu offer several scenarios for the reversal of multidrug resistance. In this study, we have observed higher sensitivity of doxorubicin-resistant uterine sarcoma MES-SA/DX5 cells to ultrasound exposure compared to its parent counterpart MES-SA cells; however, the results showed that the acoustic irradiation was genotoxic and could promote neotic division in exposed cells that was more pronounced in the resistant variant. The neotic progeny, imaged microscopically 24 hr post sonication, could contribute in modulating the final cell survival when an apoptotic dose of doxorubicin was combined with ultrasound applied either simultaneously or sequentially in dual-treatment protocols. Depending on the time and order of application of ultrasound and doxorubicin in combination treatments, there was either desensitization of the parent cells or sensitization of the resistant cells to doxorubicin action.

Original language	English
Article number	e48291
Journal	PloS one
Volume	7
Issue number	12
DOIs	https://doi.org/10.1371/journal.pone.0048291
Publication status	Published - 2012 Dec 19
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
General

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AU - Hassan, Mariame A.

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AU - Minemura, Masami

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AU - Sugiyama, Toshiro

AU - Kondo, Takashi

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AB - The acoustic effects in a biological milieu offer several scenarios for the reversal of multidrug resistance. In this study, we have observed higher sensitivity of doxorubicin-resistant uterine sarcoma MES-SA/DX5 cells to ultrasound exposure compared to its parent counterpart MES-SA cells; however, the results showed that the acoustic irradiation was genotoxic and could promote neotic division in exposed cells that was more pronounced in the resistant variant. The neotic progeny, imaged microscopically 24 hr post sonication, could contribute in modulating the final cell survival when an apoptotic dose of doxorubicin was combined with ultrasound applied either simultaneously or sequentially in dual-treatment protocols. Depending on the time and order of application of ultrasound and doxorubicin in combination treatments, there was either desensitization of the parent cells or sensitization of the resistant cells to doxorubicin action.

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Ultrasound-Induced New Cellular Mechanism Involved in Drug Resistance

Abstract

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