TY - GEN
T1 - ULTRASOUND-TRIGGERED ON-DEMAND ADENO-ASSOCIATED-VIRUS RELEASE FROM HYDROGEL MICROBEADS FOR GENE THERAPY
AU - Takatsuka, Shuhei
AU - Kubota, Takeshi
AU - Kurashina, Yuta
AU - Onoe, Hiroaki
N1 - Funding Information:
This paper was partly supported by Translational Research program; Strategic Promotion application ofInnovativemedicalTechnology(TR-SPRINT), AMED, Japan.
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - This paper reports ultrasound-triggered adeno-associated-virus (AAV) release from alginate hydrogel microbeads loaded into a gelatin hydrogel carrier to promote the transfection of cells. As a AAV carrier, caldium alginate hydrogel microbeads were centrifugally fabricated by ejecting a mixed pre-gel solution composed of sodium alginate, AAV and tungsten particles to CaCl2 solution to form ionically cross-linked instantly. For fixing of the beads, the microbeads were loaded into gelatin hydrogel carriers. The tungsten particles having high acoustic impedance enable the beads to have high sensitivity to ultrasound for improvement of the transfection. Our proposed ultrasound-triggered AAV releasing system would be an effective controlled release system for gene therapy.
AB - This paper reports ultrasound-triggered adeno-associated-virus (AAV) release from alginate hydrogel microbeads loaded into a gelatin hydrogel carrier to promote the transfection of cells. As a AAV carrier, caldium alginate hydrogel microbeads were centrifugally fabricated by ejecting a mixed pre-gel solution composed of sodium alginate, AAV and tungsten particles to CaCl2 solution to form ionically cross-linked instantly. For fixing of the beads, the microbeads were loaded into gelatin hydrogel carriers. The tungsten particles having high acoustic impedance enable the beads to have high sensitivity to ultrasound for improvement of the transfection. Our proposed ultrasound-triggered AAV releasing system would be an effective controlled release system for gene therapy.
KW - Acoustic impedance
KW - Adeno-associated-virus
KW - Gene therapy
KW - Hydrogel microbeads
KW - Ultrasound
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M3 - Conference contribution
AN - SCOPUS:85136935478
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 655
EP - 656
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -