TY - JOUR
T1 - Unexpectedly Efficient Homing Capacity of Purified Murine Hematopoietic Stem Cells
AU - Matsuzaki, Yumi
AU - Kinjo, Kentaro
AU - Mulligan, Richard C.
AU - Okano, Hideyuki
N1 - Funding Information:
We thank Drs. Toshio Suda and Seiji Okada for useful comments and discussions. We also thank Hiroko Kohike for technical assistance; and Kumiko Inoue and Akiyo Hirayama for excellent secretarial assistance. This work was supported in part by grants from Core Research for Evolutional Science and Technology (CREST) of the Japan Science and Technology Corporation; from the Ministry of Education, Science, and Culture of Japan to H.O. and Y.M.; and a grant-in-aid from the 21 st Century COE program of the Ministry of Education, Science, and Culture of Japan to Keio University.
PY - 2004/1
Y1 - 2004/1
N2 - Single-cell transplantation analysis revealed that the cells that had the strongest dye efflux activity ("Tip"-SP cells) and had the phenotype CD34- c-Kit+ Sca-1+ Lin- (CD34- KSL cells) exhibited very strong proliferation and multilineage differentiation capacity. Ninety-six percent of the lethally irradiated mice that received a single "Tip"-SP CD34- KSL cell showed significant donor cell engraftment for long term. These findings support the hypothesis that "Tip"-SP CD34- KSL cells represent the most primitive hematopoietic stem cells that are capable of migrating into the primary site and surviving and/or proliferating with nearly absolute efficiency. This led us to propose high marrow-seeding efficiency as a specific characteristic of primitive HSCs, in addition to their self-renewal and multipotent capacity.
AB - Single-cell transplantation analysis revealed that the cells that had the strongest dye efflux activity ("Tip"-SP cells) and had the phenotype CD34- c-Kit+ Sca-1+ Lin- (CD34- KSL cells) exhibited very strong proliferation and multilineage differentiation capacity. Ninety-six percent of the lethally irradiated mice that received a single "Tip"-SP CD34- KSL cell showed significant donor cell engraftment for long term. These findings support the hypothesis that "Tip"-SP CD34- KSL cells represent the most primitive hematopoietic stem cells that are capable of migrating into the primary site and surviving and/or proliferating with nearly absolute efficiency. This led us to propose high marrow-seeding efficiency as a specific characteristic of primitive HSCs, in addition to their self-renewal and multipotent capacity.
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U2 - 10.1016/S1074-7613(03)00354-6
DO - 10.1016/S1074-7613(03)00354-6
M3 - Article
C2 - 14738767
AN - SCOPUS:1642578983
SN - 1074-7613
VL - 20
SP - 87
EP - 93
JO - Immunity
JF - Immunity
IS - 1
ER -
