Up-regulation of nicotinic acetylcholine receptors by central-type acetylcholinesterase inhibitors in rat cortical neurons

Toshiaki Kume, Mitsuhiro Sugimoto, Yuki Takada, Toshiaki Yamaguchi, Atsushi Yonezawa, Hiroshi Katsuki, Hachiro Sugimoto, Akinori Akaike

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)

Abstract

We previously reported that donepezil, a central-type acetylcholinesterase inhibitor, showed neuroprotective action via α4-and α7-nicotinic acetylcholine receptors against glutamate neurotoxicity in rat cortical culture. The present study was performed to investigate whether the neuroprotective action of acetylcholinesterase inhibitors is accompanied by the alteration of expression and function of nicotinic receptors. Four days treatment with acetylcholinesterase inhibitors (10 μM) enhanced the nicotine-induced increase of the intracellular calcium concentration. Immunoblot analysis revealed that donepezil increased both α4 and α7 subunit proteins. Donepezil and galanthamine increased the number of cells expressing α4- and α7-nicotinic receptors in immunocytochemical analysis. We examined whether up-regulation of nicotinic receptors affected the neuroprotective action of acetylcholinesterase inhibitors. Under up-regulating conditions, donepezil and galanthamine exerted neuroprotective action at lower concentrations. These results suggest that donepezil and galanthamine up-regulate nicotinic receptors in cortical neurons, and that the up-regulation of nicotinic receptors may make cortical neurons more sensitive to the neuroprotective action of donepezil and galanthamine.

Original languageEnglish
Pages (from-to)77-85
Number of pages9
JournalEuropean journal of pharmacology
Volume527
Issue number1-3
DOIs
Publication statusPublished - 2005 Dec 19
Externally publishedYes

Keywords

  • Acetylcholinesterase inhibitor
  • Alzheimer's disease
  • Neuroprotection
  • Nicotinic acetylcholine receptor
  • Up-regulation

ASJC Scopus subject areas

  • Pharmacology

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