TY - GEN
T1 - USING ENCAPSULATION TO IMPROVE THE VIABILITY OF CRYOPRESERVED CELLS
AU - Matsumoto, Yoshifumi
AU - Morinaga, Yukihiro
AU - Ujihira, Masanobu
AU - Oka, Kotaro
AU - Tanishita, Kazuo
N1 - Funding Information:
This work was partially supported by a Grant-in-Aid for research for the future's program of Japan Society for the Promotion of Science.
Publisher Copyright:
© 1999 American Society of Mechanical Engineers (ASME). All rights reserved.
PY - 1999
Y1 - 1999
N2 - The purpose of this study is to clarify whether encapsulated cells have an advantage over suspended cells in cryopreservation. Rat pheochromocytoma (PC 12) cells were selected for test biological cell and microencapsulated in alginate-polylysine-alginate membranes. Microencapsulated PC12 cells were frozen with differential scanning calorimetry (DSC) at a cooling rate of 0.5 to 10°C/min, their latent heat was measured among the freezing process over the temperature range 4 to -80°C. Their post-thaw viability were evaluated by dye exclusion assay and dopamine release. As a result, latent heat of encapsulated cells was lower than that of suspended cells at a cooling rate of 0.5 and 1°C/min. This is because extra-capsule was frozen and intra-capsule unfrozen, as ice crystals forms in extra-capsule space. Post-thaw viability of microencapsulated PC 12 cells was improved at 0.5 and 1°C/min compared with that of suspended cells. Therefore, in microencapsulated PC 12 cells, achievement of intra-capsule unfrozen condition during freezing leads to reducing the solution effect and improving the viability.
AB - The purpose of this study is to clarify whether encapsulated cells have an advantage over suspended cells in cryopreservation. Rat pheochromocytoma (PC 12) cells were selected for test biological cell and microencapsulated in alginate-polylysine-alginate membranes. Microencapsulated PC12 cells were frozen with differential scanning calorimetry (DSC) at a cooling rate of 0.5 to 10°C/min, their latent heat was measured among the freezing process over the temperature range 4 to -80°C. Their post-thaw viability were evaluated by dye exclusion assay and dopamine release. As a result, latent heat of encapsulated cells was lower than that of suspended cells at a cooling rate of 0.5 and 1°C/min. This is because extra-capsule was frozen and intra-capsule unfrozen, as ice crystals forms in extra-capsule space. Post-thaw viability of microencapsulated PC 12 cells was improved at 0.5 and 1°C/min compared with that of suspended cells. Therefore, in microencapsulated PC 12 cells, achievement of intra-capsule unfrozen condition during freezing leads to reducing the solution effect and improving the viability.
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U2 - 10.1115/IMECE1999-0583
DO - 10.1115/IMECE1999-0583
M3 - Conference contribution
AN - SCOPUS:85122684287
T3 - ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
SP - 57
EP - 58
BT - Advances in Heat and Mass Transfer in Biotechnology
PB - American Society of Mechanical Engineers (ASME)
T2 - ASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999
Y2 - 14 November 1999 through 19 November 1999
ER -