TY - JOUR
T1 - Vasoactive intestinal peptide modulates T lymphocyte migration in Peyer's patches of rat small intestine
AU - Miura, Soichiro
AU - Serizawa, Hiroshi
AU - Tsuzuki, Yoshikazu
AU - Kurose, Iwao
AU - Suematsu, Makoto
AU - Higuchi, Hajime
AU - Shigematsu, Takeharu
AU - Hokari, Ryota
AU - Hirokawa, Masahiko
AU - Kimura, Hiroyuki
AU - Ishii, Hiromasa
PY - 1997/1
Y1 - 1997/1
N2 - Although vasoactive intestinal peptide (VIP) has been postulated to function in modulation of T cell trafficking, the exact mechanism has not been elucidated in vivo. In the present study, the effects of VIP on T lymphocyte migration were examined in rat Peyer's patches. T lymphocytes collected from intestinal lymph of rats were labeled with carboxyfluorescein diacetate succinimidyl ester and injected into the jugular vein. Peyer's patches of the recipient rats were observed with intravital fluorescence microscopy. In vivo intra-arterial infusion of or in vitro incubation with VIP did not affect the initial lymphocyte interaction with postcapillary venules of Peyer's patches. However, these treatments with VIP significantly inhibited transendothelial migration and also significantly blocked the interstitial migration of T cells and inhibited their subsequent appearance in the interfollicular lymphatics. Treatment with adenosine 3',5'-cyclic monophosphate (cAMP)-inducing agents resulted in similar inhibitory effect on T lymphocyte migration in Peyer's patches. In conclusion, VIP has significant inhibitory effects on T lymphocyte migration in Peyer's patches, possibly mediated by elevation of the intracellular cAMP concentrations.
AB - Although vasoactive intestinal peptide (VIP) has been postulated to function in modulation of T cell trafficking, the exact mechanism has not been elucidated in vivo. In the present study, the effects of VIP on T lymphocyte migration were examined in rat Peyer's patches. T lymphocytes collected from intestinal lymph of rats were labeled with carboxyfluorescein diacetate succinimidyl ester and injected into the jugular vein. Peyer's patches of the recipient rats were observed with intravital fluorescence microscopy. In vivo intra-arterial infusion of or in vitro incubation with VIP did not affect the initial lymphocyte interaction with postcapillary venules of Peyer's patches. However, these treatments with VIP significantly inhibited transendothelial migration and also significantly blocked the interstitial migration of T cells and inhibited their subsequent appearance in the interfollicular lymphatics. Treatment with adenosine 3',5'-cyclic monophosphate (cAMP)-inducing agents resulted in similar inhibitory effect on T lymphocyte migration in Peyer's patches. In conclusion, VIP has significant inhibitory effects on T lymphocyte migration in Peyer's patches, possibly mediated by elevation of the intracellular cAMP concentrations.
KW - adenosine 3',5'- cyclic monophosphate
KW - lymphatics
KW - postcapillary venules
KW - transendothelium
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U2 - 10.1152/ajpgi.1997.272.1.g92
DO - 10.1152/ajpgi.1997.272.1.g92
M3 - Article
C2 - 9038881
AN - SCOPUS:0031031248
SN - 0193-1857
VL - 272
SP - G92-G99
JO - American Journal of Physiology - Gastrointestinal and Liver Physiology
JF - American Journal of Physiology - Gastrointestinal and Liver Physiology
IS - 1 35-1
ER -