Very late antigen-4 in CD18-independent neutrophil emigration during acute bacterial pneumonia in mice

Sadatomo Tasaka, Sarah E. Richer, Joseph P. Mizgerd, Claire M. Doerschuk

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)


This study tested the hypothesis that very late antigen (VLA)-4 mediates CD18-independent neutrophil emigration into the air-spaces induced by either Streptococcus pneumoniae, a stimulus that induces primarily CD18-independent neutrophil emigration, or Escherichia coli, toward which only 20-30% of the total number of neutrophils emigrate through CD18-independent pathways. In wild-type (WT) mice, VLA-4 expression was less on neutrophils that emigrated into the airspaces than on circulating neutrophils. Vascular cell adhesion molecule-1 (VCAM-1) mRNA, the major endothelial cell ligand for VLA-4, increased more in E. coli than in S. pneumoniae pneumonia. VCAM-1 protein expression was not detected in capillaries, the major site of neutrophil emigration. Neutrophil emigration during E. coli or S. pneumoniae pneumonia was similar in mice given antibodies against both CD18 and VLA-4 compared with mice given the anti-CD18 antibody and a control antibody. However, in hematopoietically reconstituted mice with both WT and CD18-deficient neutrophils in their blood, the migration of CD18-deficient neutrophils in response to S. pneumoniae was slightly but significantly less in animals pretreated with the anti-VLA-4 antibody than in those receiving a control antibody. These data suggest that VLA-4 plays a small role in CD18-independent neutrophil emigration, but the majority of CD18-independent neutrophil emigration induced by bacteria in the lungs occurs through VLA-4-independent mechanisms.

Original languageEnglish
Pages (from-to)53-60
Number of pages8
JournalAmerican journal of respiratory and critical care medicine
Issue number1
Publication statusPublished - 2002 Jul 1
Externally publishedYes


  • Bacterial infection
  • Inflammation
  • Leukocyte adhesion molecules
  • Mice
  • Phagocytes

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine


Dive into the research topics of 'Very late antigen-4 in CD18-independent neutrophil emigration during acute bacterial pneumonia in mice'. Together they form a unique fingerprint.

Cite this