TY - JOUR
T1 - Wax-assisted one-step enzyme-linked immunosorbent assay on lateral flow test devices
AU - Ishii, Masanori
AU - Preechakasedkit, Pattarachaya
AU - Yamada, Kentaro
AU - Chailapakul, Orawon
AU - Suzuki, Koji
AU - Citterio, Daniel
N1 - Funding Information:
P. P. gratefully acknowledges financial support from the Thailand Research Fund (TRF) through the International Research Network (PHD58W0001), the Thailand Research Fund through Research Team Promotion Grant (RTA6080002) and the 90th Anniversary of Chulalongkorn University Fund (Ratchadphiseksomphot Endowment Fund). K. Y. kindly acknowledges the funding from a Research Fellowship of the Japan Society for the Promotion of Science (JSPS) for Young Scientists.
Publisher Copyright:
© The Japan Society for Analytical Chemistry, 2018.
PY - 2018
Y1 - 2018
N2 - Lateral flow tests (LFTs) are widely used analytical tools characterized by portability, operator simplicity and short analysis times. A remaining challenge is their limited analytical sensitivity, which in classical immunoassay formats is overcome by enzyme-linked immunosorbent assay (ELISA) formats. The implementation of ELISA to an LFT format however, is hampered by the complexity of the procedure requiring the enzyme substrate addition after sample addition. In this work, a simple method for automation of this procedure without user interference is presented. Originally used sample pads of LFTs have been replaced by hydrophobic wax-modified filter paper-based sample pads to realize a delayed flow a pre-deposited colorimetric ELISA substrate without other alterations to the classical lateral-flow immunoassay format. The performance of the system has been characterized by visualizing flow behavior and final proof-of-concept is provided by a model mouse IgG assay, achieving a limit of detection of 15.8 ng mL -1 from just a single application of the sample solution.
AB - Lateral flow tests (LFTs) are widely used analytical tools characterized by portability, operator simplicity and short analysis times. A remaining challenge is their limited analytical sensitivity, which in classical immunoassay formats is overcome by enzyme-linked immunosorbent assay (ELISA) formats. The implementation of ELISA to an LFT format however, is hampered by the complexity of the procedure requiring the enzyme substrate addition after sample addition. In this work, a simple method for automation of this procedure without user interference is presented. Originally used sample pads of LFTs have been replaced by hydrophobic wax-modified filter paper-based sample pads to realize a delayed flow a pre-deposited colorimetric ELISA substrate without other alterations to the classical lateral-flow immunoassay format. The performance of the system has been characterized by visualizing flow behavior and final proof-of-concept is provided by a model mouse IgG assay, achieving a limit of detection of 15.8 ng mL -1 from just a single application of the sample solution.
KW - ELISA
KW - Lateral flow immunoassay
KW - Sequential reagent delivery
KW - Signal amplification
KW - Wax printing
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U2 - 10.2116/analsci.34.51
DO - 10.2116/analsci.34.51
M3 - Article
C2 - 29321458
AN - SCOPUS:85041796478
SN - 0910-6340
VL - 34
SP - 51
EP - 56
JO - analytical sciences
JF - analytical sciences
IS - 1
ER -