TY - JOUR
T1 - Yb integrates piRNA intermediates and processing factors into perinuclear bodies to enhance piRISC assembly
AU - Murota, Yukiko
AU - Ishizu, Hirotsugu
AU - Nakagawa, Shinichi
AU - Iwasaki, Yuka W.
AU - Shibata, Shinsuke
AU - Kamatani, Miharu K.
AU - Saito, Kuniaki
AU - Okano, Hideyuki
AU - Siomi, Haruhiko
AU - Siomi, Mikiko C.
N1 - Funding Information:
We thank H. Masumoto for technical advice and Y. Iyoda, H. Kotani, T. Yano, and T. Nagai for technical assistance. We also thank M. Isogai and M. Shimura (PerkinElmer Japan) for their assistance with fluorescence quantification. This work was supported by CREST-JST (to M.C.S.), by a Grant-in-Aid for Scientific Research from MEXT (to S.N., Y.W.I., S.S., K.S., H.O., H.S. and M.C.S.), and by a Grant-in-Aid for the Global COE program from MEXT to Keio University (H.S. and H.O.).
PY - 2014/7/10
Y1 - 2014/7/10
N2 - PIWI-interacting RNAs (piRNAs) direct Piwi to repress transposons and maintain genome integrity in Drosophila ovarian somatic cells. piRNA maturation and association with Piwi occur at perinuclear Yb bodies, the centers of piRNA biogenesis. Here, we show that piRNA intermediates arising from the piRNA cluster flamenco ( flam) localize to perinuclear foci adjacent to Yb bodies, termed Flam bodies. RNAi-based screening of piRNA factors revealed that Flam body formation depends on Yb, the core component of Yb bodies, while Piwi and another Yb body component, Armitage, are dispensable forformation. Abolishing the RNA-binding activity of Yb disrupts both Flam bodies and Yb bodies. Yb directly binds flam, but not transcripts from neighboring protein-coding genes. Thus, Yb integrates piRNA intermediates and piRNA processing factors selectively into Flam bodies and Yb bodies, respectively. We suggest that Yb is a key upstream factor in the cytoplasmic phase of the piRNA pathway in ovarian somatic cells.
AB - PIWI-interacting RNAs (piRNAs) direct Piwi to repress transposons and maintain genome integrity in Drosophila ovarian somatic cells. piRNA maturation and association with Piwi occur at perinuclear Yb bodies, the centers of piRNA biogenesis. Here, we show that piRNA intermediates arising from the piRNA cluster flamenco ( flam) localize to perinuclear foci adjacent to Yb bodies, termed Flam bodies. RNAi-based screening of piRNA factors revealed that Flam body formation depends on Yb, the core component of Yb bodies, while Piwi and another Yb body component, Armitage, are dispensable forformation. Abolishing the RNA-binding activity of Yb disrupts both Flam bodies and Yb bodies. Yb directly binds flam, but not transcripts from neighboring protein-coding genes. Thus, Yb integrates piRNA intermediates and piRNA processing factors selectively into Flam bodies and Yb bodies, respectively. We suggest that Yb is a key upstream factor in the cytoplasmic phase of the piRNA pathway in ovarian somatic cells.
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U2 - 10.1016/j.celrep.2014.05.043
DO - 10.1016/j.celrep.2014.05.043
M3 - Article
C2 - 24953657
AN - SCOPUS:84904034861
SN - 2211-1247
VL - 8
SP - 103
EP - 113
JO - Cell Reports
JF - Cell Reports
IS - 1
ER -