@article{897939f7fe424b38a4fb285067b0e9d4,
title = "1000 spider silkomes: Linking sequences to silk physical properties",
abstract = "Spider silks are among the toughest known materials and thus provide models for renewable, biodegradable, and sustainable biopolymers. However, the entirety of their diversity still remains elusive, and silks that exceed the performance limits of industrial fibers are constantly being found. We obtained transcriptome assemblies from 1098 species of spiders to comprehensively catalog silk gene sequences and measured the mechanical, thermal, structural, and hydration properties of the dragline silks of 446 species. The combination of these silk protein genotype-phenotype data revealed essential contributions of multicomponent structures with major ampullate spidroin 1 to 3 paralogs in high-performance dragline silks and numerous amino acid motifs contributing to each of the measured properties. We hope that our global sampling, comprehensive testing, integrated analysis, and open data will provide a solid starting point for future biomaterial designs.",
author = "Kazuharu Arakawa and Nobuaki Kono and Malay, {Ali D.} and Ayaka Tateishi and Nao Ifuku and Hiroyasu Masunaga and Ryota Sato and Kousuke Tsuchiya and Rintaro Ohtoshi and Daniel Pedrazzoli and Asaka Shinohara and Yusuke Ito and Hiroyuki Nakamura and Akio Tanikawa and Yuya Suzuki and Takeaki Ichikawa and Shohei Fujita and Masayuki Fujiwara and Masaru Tomita and Blamires, {Sean J.} and Chuah, {Jo Ann} and Hamish Craig and Foong, {Choon P.} and Gabriele Greco and Juan Guan and Chris Holland and Kaplan, {David L.} and Kumar Sudesh and Mandal, {Biman B.} and Y. Norma-Rashid and Oktaviani, {Nur A.} and Preda, {Rucsanda C.} and Pugno, {Nicola M.} and Rangam Rajkhowa and Xiaoqin Wang and Kenjiro Yazawa and Zhaozhu Zheng and Keiji Numata",
note = "Funding Information: We acknowledge the MICET (especially, T. Vololontiana), the Ministry of Environment and Sustainable Development (Minist{\`e}re de l'Environnement de l'Ecologie et des For{\^e}ts at that time), the MZBA, and the University of Antananarivo for spider sampling in Madagascar. We thank Y. Takai, N. Ishii, and Y. Onozawa for technical support in sequencing; H. Ozaki and M. Sato for meaningful discussion; and H. Kano, R. Sato, and H. Nishijima for the development of reeling machines. This work was supported by grants from the ImPACT Program of Council for Science, Technology and Innovation (Cabinet Office, Government of Japan) to K.A., H.N., and K.N.; by research funds from the Yamagata Prefectural Government and Tsuruoka City, Japan, to K.A., N.K., and M.T.; and by JST ERATO grant number JPMJER1602, Grant-in-Aid for Transformative Research Areas (B), and Material DX to K.N. Publisher Copyright: Copyright {\textcopyright} 2022 The Authors, some rights reserved.",
year = "2022",
month = oct,
doi = "10.1126/sciadv.abo6043",
language = "English",
volume = "8",
journal = "Science Advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "41",
}
