TY - JOUR
T1 - 16S rRNA gene-based analysis of microbial community by whole-genome amplification and minigel-single-strand conformation polymorphism technique
AU - Oto, Michiei
AU - Suda, Wataru
AU - Shinoyama, Hirofumi
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (no. 14360197).
PY - 2006/11
Y1 - 2006/11
N2 - We have developed an analytical technique for the 16S rRNA gene that comprises whole-genome amplification and the polymerase chain reaction (PCR)-minigel-single-strand conformation polymorphism technique (WGA-SSCP). Under optimal conditions, SSCP bands could be detected when genomic DNA from bacteria of interest comprised 0.5% or more of the specimen. This method will be effective for the identification of nonculturable bacteria in a microbial community.
AB - We have developed an analytical technique for the 16S rRNA gene that comprises whole-genome amplification and the polymerase chain reaction (PCR)-minigel-single-strand conformation polymorphism technique (WGA-SSCP). Under optimal conditions, SSCP bands could be detected when genomic DNA from bacteria of interest comprised 0.5% or more of the specimen. This method will be effective for the identification of nonculturable bacteria in a microbial community.
KW - 16S rRNA gene
KW - nonculturable bacteria
KW - polymerase chain reaction (PCR)
KW - single-strand conformation polymorphism (SSCP)
KW - whole-genome amplification (WGA)
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U2 - 10.1263/jbb.102.482
DO - 10.1263/jbb.102.482
M3 - Article
C2 - 17189181
AN - SCOPUS:33845615282
SN - 1389-1723
VL - 102
SP - 482
EP - 484
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 5
ER -