@inproceedings{8e8aa7f818b146a8a89644c4b97709a8,
title = "3D fiber-shaped culture system promotes differentiation of multi potent DFAT cells into smooth muscle-like cells",
abstract = "Here we show that 3D fiber-shaped culture system enhances the differentiation of multipotent DFAT (dedifferentiated fat) cells into smooth muscle-like cells. DFAT cells cultured as 3D cell fiber constructs were induced to differentiate into smooth muscle cells. We found expression of smooth muscle lineage specific markers ASMA ( α-smooth muscle actin) and calponin in the differentiated DFAT cell fibers. The expression of calponin as marker of more mature smooth muscle cells has not been achieved in 2D cultures before. These findings suggest that 3D fiber microenvironment is able to promote more efficient differentiation into smooth muscle-like cells compared to 2D culture.",
keywords = "3D culture, Differentiation, Microfluidic, Smooth muscle",
author = "Hsiao, {A. Y.} and T. Okitsu and H. Onoe and M. Kiyosawa and H. Teramae and S. Iwanaga and S. Miura and T. Kazama and T. Matsumoto and S. Takeuchi",
year = "2013",
month = jan,
day = "1",
language = "English",
isbn = "9781632666246",
series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
publisher = "Chemical and Biological Microsystems Society",
pages = "410--412",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
note = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",
}