A characteristic N-glycopeptide signature associated with diabetic cognitive impairment identified in a longitudinal cohort study

Yuri Miura, Hiroki Tsumoto, Yukie Masui, Hiroki Inagaki, Madoka Ogawa, Yuta Ideno, Kyojiro Kawakami, Keitaro Umezawa, Mai Kabayama, Yuya Akagi, Hiroshi Akasaka, Koichi Yamamoto, Hiromi Rakugi, Tatsuro Ishizaki, Yasumichi Arai, Kazunori Ikebe, Kei Kamide, Yasuyuki Gondo, Tamao Endo

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Background: Identifying a biomarker for the decline in cognitive function in patients with diabetes is important. Therefore, we aimed to identify the N-glycopeptides on plasma proteins associated with diabetic cognitive impairment in participants in a longitudinal study using N-glycoproteomics. Methods: We used samples from the 3-year SONIC (Septuagenarians, Octogenarians, Nonagenarians Investigation with Centenarians) longitudinal cohort study of older Japanese people in the general population. First, we placed the participants with diabetes into two groups: those that did or did not have cognitive decline over a 6-year period. Next, their plasma protein profiles were compared between baseline and the 6-year time point using two-dimensional fluorescence difference gel electrophoresis. Finally, an N-glycoproteomic study of the focused proteins was performed using an enrichment technique and liquid chromatography-tandem mass spectrometry. Results: Approximately 500 N-glycopeptides, derived from 18 proteins, were identified in each sample, from among which we identified the N-glycopeptides that were associated with diabetic cognitive impairment using multivariate analysis. We found that N-glycopeptides with sialylated tri- or tetra-antennary glycans on alpha-2-macroglobulin, clusterin, serum paraoxonase/arylesterase 1, and haptoglobin were less abundant, whereas 3-sialylated tri-antennary N-glycopeptides on serotransferrin were more abundant. Conclusion: N-glycopeptides with sialylated multi-antennary glycans comprise a characteristic signature associated with diabetic cognitive impairment. General significance: The characterized N-glycopeptides represent potential biomarker candidates for diabetic cognitive impairment.

本文言語English
論文番号130316
ジャーナルBiochimica et Biophysica Acta - General Subjects
1867
4
DOI
出版ステータスPublished - 2023 4月

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学

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