TY - JOUR
T1 - A human IAP-family gene, Apollon, expressed in human brain cancer cells
AU - Chen, Zhihong
AU - Naito, Mikihiko
AU - Hori, Satoko
AU - Mashima, Tetsuo
AU - Yamori, Takao
AU - Tsuruo, Takashi
N1 - Funding Information:
We thank Drs. H. Seimiya, N. Fujita, S. Dan, H. Suzuki, and A. Tomida for helpful discussions. This study was supported in part by a special grant for Advanced Research on Cancer, Grants in Aid for Cancer Research from the Ministry of Education, Science and Culture, Japan, and the Vehicle Racing Commemorable Foundation.
PY - 1999/11/2
Y1 - 1999/11/2
N2 - IAP is a family of protein that has baculovirus IAP repeat (BIR) domains and inhibits apoptosis. We found a human IAP family gene, which we named Apollon, encoding a huge protein (530 kDa) that contains a single BIR domain and a ubiquitin-conjugating enzyme domain, that is a human homolog of BRUCE. Apollon protein was expressed in four of six brain cancers (gliomas), and one of five ovarian cancers in 38 human cancer cell lines that we examined. Among the brain cancer cell lines, SNB-78 expressed a high level of Apollon, and this cell line shows resistance against various anticancer drugs. Treating SNB-78 cells with antisense oligonucleotide against Apollon reduced the expression of Apollon protein, and significantly sensitized the cells to apoptosis induced by cisplatin and camptothecin. These results suggest that Apollon protects SNB-78 cells from undergoing apoptosis and, at least in part, plays a role in tumorigenesis and drug resistance of this cell line.
AB - IAP is a family of protein that has baculovirus IAP repeat (BIR) domains and inhibits apoptosis. We found a human IAP family gene, which we named Apollon, encoding a huge protein (530 kDa) that contains a single BIR domain and a ubiquitin-conjugating enzyme domain, that is a human homolog of BRUCE. Apollon protein was expressed in four of six brain cancers (gliomas), and one of five ovarian cancers in 38 human cancer cell lines that we examined. Among the brain cancer cell lines, SNB-78 expressed a high level of Apollon, and this cell line shows resistance against various anticancer drugs. Treating SNB-78 cells with antisense oligonucleotide against Apollon reduced the expression of Apollon protein, and significantly sensitized the cells to apoptosis induced by cisplatin and camptothecin. These results suggest that Apollon protects SNB-78 cells from undergoing apoptosis and, at least in part, plays a role in tumorigenesis and drug resistance of this cell line.
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U2 - 10.1006/bbrc.1999.1585
DO - 10.1006/bbrc.1999.1585
M3 - Article
C2 - 10544019
AN - SCOPUS:0033517839
SN - 0006-291X
VL - 264
SP - 847
EP - 854
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -
