TY - GEN
T1 - A microfluidic invasion assay for glioma-initiating cells in three-dimensional culture
AU - Fujioka, Shingo
AU - Oltea, Sampetrean
AU - Saya, Hideyuki
AU - Sudo, Ryo
PY - 2013
Y1 - 2013
N2 - This paper presents a microfluidic three-dimensional (3D) platform to investigate behaviors of glioma-initiating cells (GIC) cocultured with human umbilical vein endothelial cells (HUVEC). In this platform, these cells were cultured in microenvironments similar to in vivo. Especially, our experiments have been performed with GIC established by gene transfection [1], which is different from conventional tumor cell lines. Our results demonstrate that biochemical factors secreted by HUVEC attracts glioma invasion.
AB - This paper presents a microfluidic three-dimensional (3D) platform to investigate behaviors of glioma-initiating cells (GIC) cocultured with human umbilical vein endothelial cells (HUVEC). In this platform, these cells were cultured in microenvironments similar to in vivo. Especially, our experiments have been performed with GIC established by gene transfection [1], which is different from conventional tumor cell lines. Our results demonstrate that biochemical factors secreted by HUVEC attracts glioma invasion.
KW - Glioma-initiating cells
KW - Invasion
KW - Microfluidic device
UR - http://www.scopus.com/inward/record.url?scp=84907379465&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907379465&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84907379465
SN - 9781632666246
T3 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
SP - 1075
EP - 1077
BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PB - Chemical and Biological Microsystems Society
T2 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Y2 - 27 October 2013 through 31 October 2013
ER -