TY - JOUR
T1 - A noncanonical endocytic pathway is involved in the internalization of 3 μm polystyrene beads into HeLa cells
AU - Hirose, Hisaaki
AU - Maekawa, Masashi
AU - Ida, Hiroki
AU - Kuriyama, Masashi
AU - Takahashi, Yasufumi
AU - Futaki, Shiroh
N1 - Funding Information:
We thank Masachika Shudou at the Division of Medical Research Support, the Advanced Research Support Center (ADRES), Ehime University for his technical assistance of scanning electron microscopy. This work was supported by JSPS KAKENHI Grant Numbers JP20K22705 and JP22K05313 to H. H., and JST CREST (Grant Number JPMJCR18H5) to S. F. This work was supported by the International Collaborative Research Program of Institute for Chemical Research, Kyoto University (Grant # 2021-112 to M. M.).
Publisher Copyright:
© 2022 The Royal Society of Chemistry.
PY - 2022
Y1 - 2022
N2 - Extracellular fine particles of various sizes and origins can be taken up by cells, affecting their function. Understanding the cellular uptake processes is crucial for understanding the cellular effects of these particles and the development of means to control their internalization. Although macropinocytosis is a possible pathway for the cellular uptake of particles larger than 0.2 μm, its contribution to cellular uptake in non-phagocytic cells is controversial. Using 3 μm polystyrene beads as a model particle, we aimed to assess the detailed modes of their cellular uptake by non-phagocytic HeLa cells. Cellular uptake was assessed using confocal, scanning electron, and scanning ion conductance microscopy analyses, together with inhibitor studies. Our results revealed that 3 μm beads were taken up by HeLa cells by an actin-, cholesterol-, and membrane protrusions-dependent noncanonical endocytic pathway, different from the canonical macropinocytic and phagocytic pathways. Our work provides a framework for studying the cellular uptake of extracellular fine particles.
AB - Extracellular fine particles of various sizes and origins can be taken up by cells, affecting their function. Understanding the cellular uptake processes is crucial for understanding the cellular effects of these particles and the development of means to control their internalization. Although macropinocytosis is a possible pathway for the cellular uptake of particles larger than 0.2 μm, its contribution to cellular uptake in non-phagocytic cells is controversial. Using 3 μm polystyrene beads as a model particle, we aimed to assess the detailed modes of their cellular uptake by non-phagocytic HeLa cells. Cellular uptake was assessed using confocal, scanning electron, and scanning ion conductance microscopy analyses, together with inhibitor studies. Our results revealed that 3 μm beads were taken up by HeLa cells by an actin-, cholesterol-, and membrane protrusions-dependent noncanonical endocytic pathway, different from the canonical macropinocytic and phagocytic pathways. Our work provides a framework for studying the cellular uptake of extracellular fine particles.
UR - https://www.scopus.com/pages/publications/85141920191
UR - https://www.scopus.com/pages/publications/85141920191#tab=citedBy
U2 - 10.1039/d2bm01353c
DO - 10.1039/d2bm01353c
M3 - Article
C2 - 36326722
AN - SCOPUS:85141920191
SN - 2047-4830
VL - 50
JO - Biomaterials Science
JF - Biomaterials Science
ER -