@article{300e3e96ea954ea391e8c350808340cf,
title = "A subtype of opioid κ-receptor is coupled to inhibition of Gil-mediated phospholipase C activity in the guinea pig cerebellum",
abstract = "PLC activity was stimulated either by 1-100 μM of GTP or by 100-3,000 μM Ca2+ in lysed synaptosomal membranes of the guinea pig cerebellum. The κ-opioid receptor agonist selectively inhibited the PLC activity stimulated by 100 μM GTP, but not by 100-3,000 μM Ca2+. Pretreatment of membranes with PTX abolished such a κ-agonist-induced inhibition of PLC activity. The reconstitution of Gi1, but not of Go purified from porcine brains with PTX-treated membranes showed a complete recovery of the κ-agonist-inhibition of PLC activity. These findings suggest that a novel subtype κ-receptor mediates inhibition of PLC through inhibiting the intrinsic activity of PTX-substrate G-proteins.",
keywords = "Gi1-type G-protein, Opioid κ-receptor, Phospholipase C",
author = "Hidemi Misawa and Hiroshi Ueda and Toshiaki Katada and Michio Ui and Masamichi Satoh",
note = "Funding Information: Acknowledgments.{"} This work has been done at the Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University. Parts of this study were supported by research grants from the Ministry of Education, Science and Culture, Japan and from Uehara Memorial Foundation, Kowa Life Science Foundation, Terumo Life Science Foundation Research Foundation for Pharmaceutical Sciences and Kato Memorial Foundation.",
year = "1995",
month = mar,
day = "13",
doi = "10.1016/0014-5793(95)00162-3",
language = "English",
volume = "361",
pages = "106--110",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1",
}