Accelerated cartilage resorption by chondroclasts during bone fracture healing in osteoprotegerin-deficient mice

Norikazu Ota, Hironari Takaishi, Naoto Kosaki, Jiro Takito, Masaki Yoda, Takahide Tohmonda, Tokuhiro Kimura, Yasunori Okada, Hisataka Yasuda, Hiroshi Kawaguchi, Morio Matsumoto, Kazuhiro Chiba, Hiroyasu Ikegami, Yoshiaki Toyama

研究成果: Article査読

37 被引用数 (Scopus)


Receptor activator of nuclear factor-κB ligand (RANKL) and osteoprotegerin (OPG), a decoy receptor of RANKL, maintain bone mass by regulating the differentiation of osteoclasts, which are bone-resorbing cells. Endochondral bone ossification and bone fracture healing involve cartilage resorption, a less well-understood process that is needed for replacement of cartilage by bone. Here we describe the role of OPG produced by chondrocytes in chondroclastogenesis. Fracture healing in OPG-/- mice showed faster union of the fractured bone, faster resorption of the cartilaginous callus, and an increased number of chondroclasts at the chondroosseous junctions compared with that in wild-type littermates. When a cultured pellet of OPG-/- chondrocytes was transplanted beneath the kidney capsule, the pellet recruited many chondroclasts. The pellet showed the ability to induce tartrate-resistant acid phosphatase-positive multinucleated cells from RAW 264.7 cells in vitro. Finally, OPG-/- chondrocytes (but not wild-type chondrocytes) cultured with spleen cells induced many tartrate-resistant acid phosphatase-positive multinucleated cells. The expression of RANKL and OPG in chondrocytes was regulated by several osteotropic factors including 1,25-dihydroxyvitamin D3, PTHrP, IL-1α, and TNF-α. Thus, local OPG produced by chondrocytes probably controls cartilage resorption as a negative regulator for chondrocyte-dependent chondroclastogenesis.

出版ステータスPublished - 2009 11月

ASJC Scopus subject areas

  • 内分泌学


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