Activation of apoptosis by caspase-3-dependent specific RelB cleavage in anticancer agent-treated cancer cells: Involvement of positive feedback mechanism

Mizuki Kuboki; Ayumi Ito; Siro Simizu; Kazuo Umezawa

doi:10.1016/j.bbrc.2014.12.024

Activation of apoptosis by caspase-3-dependent specific RelB cleavage in anticancer agent-treated cancer cells: Involvement of positive feedback mechanism

Mizuki Kuboki, Ayumi Ito, Siro Simizu, Kazuo Umezawa

応用化学科

研究成果: Article › 査読

7 被引用数 (Scopus)

抄録

DTCM-glutarimide (DTCM-G) is a newly found anti-inflammatory agent. In the course of experiments with lymphoma cells, we found that DTCM-G induced specific RelB cleavage. Anticancer agent vinblastine also induced the specific RelB cleavage in human fibrosarcoma HT1080 cells. The site-directed mutagenesis analysis revealed that the Asp205 site in RelB was specifically cleaved possibly by caspase-3 in vinblastine-treated HT1080 cells. Moreover, the cells stably overexpressing RelB Asp205Ala were resistant to vinblastine-induced apoptosis. Thus, the specific Asp205 cleavage of RelB by caspase-3 would be involved in the apoptosis induction by anticancer agents, which would provide the positive feedback mechanism.

本文言語	English
ページ（範囲）	810-814
ページ数	5
ジャーナル	Biochemical and Biophysical Research Communications
巻	456
号	3
DOI	https://doi.org/10.1016/j.bbrc.2014.12.024
出版ステータス	Published - 2015 1月 16

ASJC Scopus subject areas

生物理学
生化学
分子生物学
細胞生物学

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

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10.1016/j.bbrc.2014.12.024

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引用スタイル

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title = "Activation of apoptosis by caspase-3-dependent specific RelB cleavage in anticancer agent-treated cancer cells: Involvement of positive feedback mechanism",

abstract = "DTCM-glutarimide (DTCM-G) is a newly found anti-inflammatory agent. In the course of experiments with lymphoma cells, we found that DTCM-G induced specific RelB cleavage. Anticancer agent vinblastine also induced the specific RelB cleavage in human fibrosarcoma HT1080 cells. The site-directed mutagenesis analysis revealed that the Asp205 site in RelB was specifically cleaved possibly by caspase-3 in vinblastine-treated HT1080 cells. Moreover, the cells stably overexpressing RelB Asp205Ala were resistant to vinblastine-induced apoptosis. Thus, the specific Asp205 cleavage of RelB by caspase-3 would be involved in the apoptosis induction by anticancer agents, which would provide the positive feedback mechanism.",

keywords = "Apoptosis, Caspase-3, DTCM-glutarimide, RelB, Vinblastine",

author = "Mizuki Kuboki and Ayumi Ito and Siro Simizu and Kazuo Umezawa",

note = "Funding Information: This work was supported financially in part by Grant-in-Aid for Scientific Research (B, 23310163 and 24310167) and (C, 26350975) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan. This work was also supported in part by MEXT-Supported Program for the Strategic Research Foundation at Private Universities, which is for Aichi Medical University 2011–2015 (S1101027). Publisher Copyright: {\textcopyright} 2015 Elsevier Inc. All rights reserved.",

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AU - Kuboki, Mizuki

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AU - Simizu, Siro

AU - Umezawa, Kazuo

N1 - Funding Information: This work was supported financially in part by Grant-in-Aid for Scientific Research (B, 23310163 and 24310167) and (C, 26350975) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan. This work was also supported in part by MEXT-Supported Program for the Strategic Research Foundation at Private Universities, which is for Aichi Medical University 2011–2015 (S1101027). Publisher Copyright: © 2015 Elsevier Inc. All rights reserved.

PY - 2015/1/16

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N2 - DTCM-glutarimide (DTCM-G) is a newly found anti-inflammatory agent. In the course of experiments with lymphoma cells, we found that DTCM-G induced specific RelB cleavage. Anticancer agent vinblastine also induced the specific RelB cleavage in human fibrosarcoma HT1080 cells. The site-directed mutagenesis analysis revealed that the Asp205 site in RelB was specifically cleaved possibly by caspase-3 in vinblastine-treated HT1080 cells. Moreover, the cells stably overexpressing RelB Asp205Ala were resistant to vinblastine-induced apoptosis. Thus, the specific Asp205 cleavage of RelB by caspase-3 would be involved in the apoptosis induction by anticancer agents, which would provide the positive feedback mechanism.

AB - DTCM-glutarimide (DTCM-G) is a newly found anti-inflammatory agent. In the course of experiments with lymphoma cells, we found that DTCM-G induced specific RelB cleavage. Anticancer agent vinblastine also induced the specific RelB cleavage in human fibrosarcoma HT1080 cells. The site-directed mutagenesis analysis revealed that the Asp205 site in RelB was specifically cleaved possibly by caspase-3 in vinblastine-treated HT1080 cells. Moreover, the cells stably overexpressing RelB Asp205Ala were resistant to vinblastine-induced apoptosis. Thus, the specific Asp205 cleavage of RelB by caspase-3 would be involved in the apoptosis induction by anticancer agents, which would provide the positive feedback mechanism.

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