抄録
Wavefront distortion in temporal focusing microscopy (TFM) results in a distorted temporal profile of the excitation pulses owing to spatio-temporal coupling. Since the pulse duration is dramatically changed in the excitation volume, it is difficult to correct the temporal profile for a thick sample. Here, we demonstrate adaptive optics (AO) correction in a thick sample. We apply structured illumination microscopy (SIM) to an AO correction in wide-field TFM to decrease the change in the pulse duration in the signal detection volume. The AO correction with SIM was very successful in a thick sample for which AO correction with TFM failed.
本文言語 | English |
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ページ(範囲) | 29021-29033 |
ページ数 | 13 |
ジャーナル | Optics Express |
巻 | 29 |
号 | 18 |
DOI | |
出版ステータス | Published - 2021 8月 30 |
ASJC Scopus subject areas
- 原子分子物理学および光学