Adipogenic differentiation of human induced pluripotent stem cells: Comparison with that of human embryonic stem cells

Daisuke Taura, Michio Noguchi, Masakatsu Sone, Kiminori Hosoda, Eisaku Mori, Yohei Okada, Kazutoshi Takahashi, Koichiro Homma, Naofumi Oyamada, Megumi Inuzuka, Takuhiro Sonoyama, Ken Ebihara, Naohisa Tamura, Hiroshi Itoh, Hirofumi Suemori, Norio Nakatsuji, Hideyuki Okano, Shinya Yamanaka, Kazuwa Nakao

研究成果: Article査読

126 被引用数 (Scopus)

抄録

Induced pluripotent stem (iPS) cells were recently established from human fibroblasts. In the present study we investigated the adipogenic differentiation properties of four human iPS cell lines and compared them with those of two human embryonic stem (ES) cell lines. After 12 days of embryoid body formation and an additional 10 days of differentiation on Poly-l-ornithine and fibronectin- coated dishes with adipogenic differentiation medium, human iPS cells exhibited lipid accumulation and transcription of adipogenesis-related molecules such as C/EBPα, PPARγ2, leptin and aP2. These results demonstrate that human iPS cells have an adipogenic potential comparable to human ES cells.

本文言語English
ページ(範囲)1029-1033
ページ数5
ジャーナルFEBS Letters
583
6
DOI
出版ステータスPublished - 2009 3月 18

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

フィンガープリント

「Adipogenic differentiation of human induced pluripotent stem cells: Comparison with that of human embryonic stem cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル