TY - GEN
T1 - Albumin uptake into endothelial cells in separated flow
AU - Kudo, Susumu
AU - Yamaguchi, Ryuhei
AU - Sato, Masashi
AU - Oka, Kotaro
AU - Tanishita, Kazuo
N1 - Funding Information:
This study was partially supported by the Grant-in-Aid for Encouragement of Young Scientists (11780629) from Japan Society for the Promotion of Science and by CASIO Science Promotion Foundation.
Publisher Copyright:
© ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE).
PY - 2000
Y1 - 2000
N2 - The purpose of this study is to reveal the albumin uptake intoendothelial cells in the separated flow area. After 24 hr of exposure toflow induced in a back step flow channel, the endothelial cells wereincubated in 371C for 60 minutes in PBS containingtetramethylrhodamine isothiocyanate conjugated albumin (TRITCalbumin). Thereafter, the cell morphology and the albumin uptakewere observed by a confocal laser scanning microscope (CLSM). In alow shear stress area (stagnant and reattachment areas), the cellsaligned randomly. In a high shear stress area (reversal and fullydeveloped areas), the cells were elongated and aligned to flowdirection. In low-shear-stress and high-shear-stress gradient areas(reattachment areas), the amount of albumin uptake into the cells wasthe largest in all areas. These data indicate that shear stress and shearstress gradient affect the endothelial cell morphology and the albuminuptake into endothelial cells.
AB - The purpose of this study is to reveal the albumin uptake intoendothelial cells in the separated flow area. After 24 hr of exposure toflow induced in a back step flow channel, the endothelial cells wereincubated in 371C for 60 minutes in PBS containingtetramethylrhodamine isothiocyanate conjugated albumin (TRITCalbumin). Thereafter, the cell morphology and the albumin uptakewere observed by a confocal laser scanning microscope (CLSM). In alow shear stress area (stagnant and reattachment areas), the cellsaligned randomly. In a high shear stress area (reversal and fullydeveloped areas), the cells were elongated and aligned to flowdirection. In low-shear-stress and high-shear-stress gradient areas(reattachment areas), the amount of albumin uptake into the cells wasthe largest in all areas. These data indicate that shear stress and shearstress gradient affect the endothelial cell morphology and the albuminuptake into endothelial cells.
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U2 - 10.1115/IMECE2000-2526
DO - 10.1115/IMECE2000-2526
M3 - Conference contribution
AN - SCOPUS:85119719907
T3 - ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
SP - 83
EP - 84
BT - Advances in Bioengineering
PB - American Society of Mechanical Engineers (ASME)
T2 - ASME 2000 International Mechanical Engineering Congress and Exposition, IMECE 2000
Y2 - 5 November 2000 through 10 November 2000
ER -