@inbook{5b6e45820efd4265989510df2fbbf2cc,
title = "Amyloid β (Aβ) ELISA of Human iPSC-Derived Neuronal Cultures",
abstract = "Amyloid β (Aβ) peptides are the main component of the characteristic insoluble deposits in brain parenchyma and small blood vessels in the patients afflicted with Alzheimer{\textquoteright}s disease (AD) and cerebral amyloid angiopathy (CAA). These small peptides are attributed to the pathogenesis of both AD and CAA, suggesting an important index for disease stage and progression. In the brain tissue, Aβs are released mainly from neuronal cells into extracellular space. Here, we describe a step-by-step protocol to measure Aβs secreted from human pluripotent stem cell-derived neuronal cells.",
keywords = "Amyloid β (Aβ), Enzyme-Linked Immuno Sorbent Assay (ELISA), Neuronal differentiation, induced pluripotent stem cell (iPSC), β-Secretase, γ-Secretase",
author = "Hirotaka Watanabe and Rei Murakami and Hideyuki Okano",
note = "Funding Information: This work was supported by funding from the Japan Society for the Promotion of Science KAKENHI (grant nos. 15H06587 and 17K08668 to H.W.) and the Research Project for Practical Applications of Regenerative Medicine from the Japan Agency for Medical Research and Development (AMED) (grant nos. 15bk0104027h0003, 16bk0104016h0004, and 17bk0104016h0005 to H.O.), the Research Center Network for Realization Research Centers/Projects of Regenerative Medicine (the Program for Intractable Disease Research Utilizing Disease- specific iPS Cells and the Acceleration Program for Intractable Diseases Research Utilizing Disease-specific iPS Cells) from AMED (grant nos. JP15bm0609003, JP16bm0609003, JP17bm0804003, JP18bm0804003, JP19bm0804003 and JP20bm0804003 and JP21bm0804003 to H.O.). We thank Dr. Mitsuru Ishikawa and Ms. Misato Sho (Keio University) for their kind support and technical advice. Funding Information: This work was supported by funding from the Japan Society for the Promotion of Science KAKENHI (grant nos. 15H06587 and 17K08668 to H.W.) and the Research Project for Practical Applications of Regenerative Medicine from the Japan Agency for Medical Research and Development (AMED) (grant nos. 15bk0104027h0003, 16bk0104016h0004, and 17bk0104016h0005 to H.O.), the Research Center Network for Realization Research Centers/Projects of Regenerative Medicine (the Program for Intractable Disease Research Utilizing Disease-specific iPS Cells and the Acceleration Program for Intractable Diseases Research Utilizing Disease-specific iPS Cells) from AMED (grant nos. JP15bm0609003, JP16bm0609003, JP17bm0804003, JP18bm0804003, JP19bm0804003 and JP20bm0804003 and JP21bm0804003 to H.O.). We thank Dr. Mitsuru Ishikawa and Ms. Misato Sho (Keio University) for their kind support and technical advice. Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC.",
year = "2022",
doi = "10.1007/7651_2021_407",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "209--217",
booktitle = "Methods in Molecular Biology",
}
