An ex vivo choroid sprouting assay of ocular microvascular angiogenesis

Yohei Tomita, Zhuo Shao, Bertan Cakir, Yumi Kotoda, Zhongjie Fu, Lois E.H. Smith

研究成果: Article査読

8 被引用数 (Scopus)

抄録

Pathological choroidal angiogenesis, a salient feature of age-related macular degeneration, leads to vision impairment and blindness. Endothelial cell (EC) proliferation assays using human retinal microvascular endothelial cells (HRMECs) or isolated primary retinal ECs are widely used in vitro models to study retinal angiogenesis. However, isolating pure murine retinal endothelial cells is technically challenging and retinal ECs may have different proliferation responses than choroidal endothelial cells and different cell/cell interactions. A highly reproducible ex vivo choroidal sprouting assay as a model of choroidal microvascular proliferation was developed. This model includes the interaction between choroid vasculature (EC, macrophages, pericytes) and retinal pigment epithelium (RPE). Mouse RPE/choroid/ scleral explants are isolated and incubated in growth-factor-reduced basal membrane extract (BME) (day 0). Medium is changed every other day and choroid sprouting is quantified at day 6. The images of individual choroid explant are taken with an inverted phase microscope and the sprouting area is quantified using a semi-automated macro plug-in to the ImageJ software developed in this lab. This reproducible ex vivo choroidal sprouting assay can be used to assess compounds for potential treatment and for microvascular disease research to assess pathways involved in choroidal micro vessel proliferation using wild type and genetically modified mouse tissue.

本文言語English
論文番号e61677
ページ(範囲)1-10
ページ数10
ジャーナルJournal of Visualized Experiments
2020
162
DOI
出版ステータスPublished - 2020 8月
外部発表はい

ASJC Scopus subject areas

  • 神経科学一般
  • 化学工学一般
  • 生化学、遺伝学、分子生物学一般
  • 免疫学および微生物学一般

フィンガープリント

「An ex vivo choroid sprouting assay of ocular microvascular angiogenesis」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル