Analysis of melatonin using a pH- and temperature-responsive aqueous chromatography system

Eri Ayano, Yusuke Suzuki, Masahito Kanezawa, Chikako Sakamoto, Yuko Morita-Murase, Yoshiko Nagata, Hideko Kanazawa, Akihiko Kikuchi, Teruo Okano

研究成果: Article査読

25 被引用数 (Scopus)


A new method for the qualitative and quantitative analysis of an intracerebral hormone, such as melatonin, has been proposed, utilizing newly designed copolymers that include ion-exchange groups. These copolymers responded to both the temperature and the pH, and the copolymers were modified with cross-linked hydrogel applied onto aminopropyl silica beads. The products were evaluated as HPLC packing materials for a pH- and temperature-responsive chromatography. The property of the surface of the stationary phase was altered from hydrophilic to hydrophobic, and from charged to non-charged by changes in both the temperature and the pH. In the chromatographic system, we investigated how to change the retention of melatonin by varying the temperature. A pH- and temperature-responsive chromatography is expected to be useful for the separation of pharmaceuticals and biomolecules.

ジャーナルJournal of Chromatography A
1-2 SPEC. ISS.
出版ステータスPublished - 2007 7月 13

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 有機化学


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