Antibody Clicking as a Strategy to Modify Antibody Functionalities on the Surface of Targeted Cells

Toru Komatsu, Etsu Kyo, Haruki Ishii, Kyoji Tsuchikama, Aiko Yamaguchi, Tasuku Ueno, Kenjiro Hanaoka, Yasuteru Urano

研究成果: Article査読

9 被引用数 (Scopus)

抄録

We established a methodology for initiating cross-linking of antibodies selectively on the cell surface through intermolecular copper-free click reactions facilitated by increased effective concentrations of antibodies binding to target antigens. Upon cross-linking of tetrazine- and bicyclononyne-modified trastuzumab on the surface of HER2-overexpressing cells, increased antibody uptake and activation of intracellular signaling were observed. Our findings demonstrate that the cross-linking reaction can significantly alter the biophysical properties of proteins, activating their unique functionalities on targeted cells to realize an increased cargo delivery and synthetic manipulation of cellular signaling.

本文言語English
ページ(範囲)15644-15648
ページ数5
ジャーナルJournal of the American Chemical Society
142
37
DOI
出版ステータスPublished - 2020 9月 16
外部発表はい

ASJC Scopus subject areas

  • 触媒
  • 化学一般
  • 生化学
  • コロイド化学および表面化学

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