Application of High-Resolution Single-Channel Recording to Functional Studies of Cystic Fibrosis Mutants

Zhiwei Cai, Yoshiro Sohma, Silvia G. Bompadre, David N. Sheppard, Tzyh Chang Hwang

研究成果: Chapter

12 被引用数 (Scopus)

抄録

The patch-clamp technique is a powerful and versatile method to investigate the cystic fibrosis transmembrane conductance regulator (CFTR) Cl channel, its malfunction in disease and modulation by small molecules. Here, we discuss how the molecular behaviour of CFTR is investigated using high-resolution single-channel recording and kinetic analyses of channel gating. We review methods used to quantify how cystic fibrosis (CF) mutants perturb the biophysical properties and regulation of CFTR. By explaining the relationship between macroscopic and single-channel currents, we demonstrate how single-channel data provide molecular explanations for changes in CFTR-mediated transepithelial ion transport elicited by CF mutants.

本文言語English
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ419-441
ページ数23
DOI
出版ステータスPublished - 2011
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
741
ISSN(印刷版)1064-3745
ISSN(電子版)1940-6029

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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