Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport

Takuya Terai; Moe Kohno; Gaelle Boncompain; Shigeru Sugiyama; Nae Saito; Ryo Fujikake; Tasuku Ueno; Toru Komatsu; Kenjiro Hanaoka; Takayoshi Okabe; Yasuteru Urano; Franck Perez; Tetsuo Nagano

doi:10.1021/jacs.5b05672

Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport

Takuya Terai
, Moe Kohno
, Gaelle Boncompain
, Shigeru Sugiyama
, Nae Saito
, Ryo Fujikake
, Tasuku Ueno
, Toru Komatsu
, Kenjiro Hanaoka
, Takayoshi Okabe
, Yasuteru Urano
, Franck Perez
, Tetsuo Nagano

研究成果: Article › 査読

22 被引用数 (Scopus)

抄録

Artificial ligands of streptavidin (ALiS) with association constants of 10⁶ M^-1 were discovered by high-throughput screening of our chemical library, and their binding characteristics, including X-ray crystal structure of the streptavidin complex, were determined. Unlike biotin and its derivatives, ALiS exhibits fast dissociation kinetics and excellent cell permeability. The streptavidin-ALiS system provides a novel, practical compound-dependent methodology for repeated reversible cycling of protein localization between intracellular organella.

本文言語	English
ページ（範囲）	10464-10467
ページ数	4
ジャーナル	Journal of the American Chemical Society
巻	137
号	33
DOI	https://doi.org/10.1021/jacs.5b05672
出版ステータス	Published - 2015 8月 11
外部発表	はい

ASJC Scopus subject areas

触媒
化学一般
生化学
コロイド化学および表面化学

文献へのアクセス

10.1021/jacs.5b05672

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引用スタイル

Terai, T., Kohno, M., Boncompain, G., Sugiyama, S., Saito, N., Fujikake, R., Ueno, T., Komatsu, T., Hanaoka, K., Okabe, T., Urano, Y., Perez, F., & Nagano, T. (2015). Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport. Journal of the American Chemical Society, 137(33), 10464-10467. https://doi.org/10.1021/jacs.5b05672

Terai, T, Kohno, M, Boncompain, G, Sugiyama, S, Saito, N, Fujikake, R, Ueno, T, Komatsu, T, Hanaoka, K, Okabe, T, Urano, Y, Perez, F & Nagano, T 2015, 'Artificial Ligands of Streptavidin (ALiS): Discovery, Characterization, and Application for Reversible Control of Intracellular Protein Transport', Journal of the American Chemical Society, vol. 137, no. 33, pp. 10464-10467. https://doi.org/10.1021/jacs.5b05672

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