TY - JOUR
T1 - Assembly of human organs from stem cells to study liver disease
AU - Handa, Kan
AU - Matsubara, Kentaro
AU - Fukumitsu, Ken
AU - Guzman-Lepe, Jorge
AU - Watson, Alicia
AU - Soto-Gutierrez, Alejandro
N1 - Funding Information:
Supported by NIH grants DK083556 (A.S.-G.) and EB0010216 (A.W.), an American Society of Transplantation Basic Science Faculty Development grant (A.S.-G.), and the Competitive Medical Research Fund of the University of Pittsburgh Medical Center Health System grant (A.S.-G.).
PY - 2014/2
Y1 - 2014/2
N2 - Recently, significant developments in the field of liver tissue engineering have raised new possibilities for the study of complex physiological and pathophysiological processes in vitro, as well as the potential to assemble entire organs for transplantation. Human-induced pluripotent stem cells have been differentiated into relatively functional populations of hepatic cells, and novel techniques to generate whole organ acellular three-dimensional scaffolds have been developed. In this review, we highlight the most recent advances in organ assembly regarding the development of liver tissue in vitro. We emphasize applications that involve multiple types of cells with a biomimetic spatial organization for which three-dimensional configurations could be used for drug development or to explain mechanisms of disease. We also discuss applications of liver organotypic surrogates and the challenges of translating the highly promising new field of tissue engineering into a proven platform for predicting drug metabolism and toxicity.
AB - Recently, significant developments in the field of liver tissue engineering have raised new possibilities for the study of complex physiological and pathophysiological processes in vitro, as well as the potential to assemble entire organs for transplantation. Human-induced pluripotent stem cells have been differentiated into relatively functional populations of hepatic cells, and novel techniques to generate whole organ acellular three-dimensional scaffolds have been developed. In this review, we highlight the most recent advances in organ assembly regarding the development of liver tissue in vitro. We emphasize applications that involve multiple types of cells with a biomimetic spatial organization for which three-dimensional configurations could be used for drug development or to explain mechanisms of disease. We also discuss applications of liver organotypic surrogates and the challenges of translating the highly promising new field of tissue engineering into a proven platform for predicting drug metabolism and toxicity.
UR - http://www.scopus.com/inward/record.url?scp=84892152296&partnerID=8YFLogxK
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U2 - 10.1016/j.ajpath.2013.11.003
DO - 10.1016/j.ajpath.2013.11.003
M3 - Review article
C2 - 24333262
AN - SCOPUS:84892152296
SN - 0002-9440
VL - 184
SP - 348
EP - 357
JO - American Journal of Pathology
JF - American Journal of Pathology
IS - 2
ER -
