Association between G2-phase block and repair of radiation-induced chromosome fragments in human lymphocytes

Marco Durante, Yoshiya Furusawa, Hideyuki Majima, Testsuya Kawata, Eisuke Gotoh

研究成果: Article査読

71 被引用数 (Scopus)

抄録

We have studied the induction of chromosomal aberrations in human lymphocytes exposed in Go to X rays or carbon ions. Aberrations were analyzed in G0, G1, G2 or M phase. Analysis during the interphase was performed by chemically induced premature chromosome condensation, which allows scoring of aberrations in G1, G2 and M phase; fusion-induced premature chromosome condensation was used to analyze the damage in Go cells after incubation for repair; M-phase cells were obtained by conventional Colcemid block. Aberrations were scored by Giemsa staining or fluorescence in situ hybridization (chromosomes 2 and 4). Similar yields of fragments were observed in G1 and G2 phase, but lower yields were scored in metaphase. The frequency of chromosomal exchanges was similar in G0 (after repair), G2 and M phase for cells exposed to X rays, while a lower frequency of exchanges was observed in M phase when lymphocytes were irradiated with high-LET carbon ions. The results suggest that radiation-induced G2-phase block is associated with unrejoined chromosome fragments induced by radiation exposure during G0.

本文言語English
ページ(範囲)670-676
ページ数7
ジャーナルRadiation Research
151
6
DOI
出版ステータスPublished - 1999 6月 1

ASJC Scopus subject areas

  • 生物理学
  • 放射線
  • 放射線学、核医学およびイメージング

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