@article{8d4ac1c72455441abeced672953adfa4,
title = "Astrocytic d-amino acid oxidase degrades d-serine in the hindbrain",
abstract = "d-Serine modulates excitatory neurotransmission by binding to N-methyl-d-aspartate glutamate receptors. d-Amino acid oxidase (DAO) degrades d-amino acids, such as d-serine, in the central nervous system, and is associated with neurological and psychiatric disorders. However, cell types that express brain DAO remain controversial, and whether brain DAO influences systemic d-amino acids in addition to brain d-serine remains unclear. Here, we created astrocyte-specific DAO-conditional knockout mice. Knockout in glial fibrillary acidic protein-positive cells eliminated DAO expression in the hindbrain and increased d-serine levels significantly in the cerebellum. Brain DAO did not influence levels of d-amino acids in the forebrain or periphery. These results show that astrocytic DAO regulates d-serine specifically in the hindbrain.",
keywords = "chirality, d-alanine, d-amino acid oxidase, d-serine",
author = "Yusuke Gonda and Chiharu Ishii and Masashi Mita and Naoto Nishizaki and Yoshiyuki Ohtomo and Kenji Hamase and Toshiaki Shimizu and Jumpei Sasabe",
note = "Funding Information: This research was funded by Japan Agency for Medical Research and Development (AMED-PRIME) (19gm6010017h0002) (JS), JSPS KAKENHI Grant number 21H02982 (JS), Shiseido. Co. Ltd. and Keio Gijuku Fukuzawa Memorial Fund for the Advancement of Education and Research (JS). We thank Hiroshi Imoto, Tatsuhiko Ikeda, Eiichi Negishi, Shoto Ishigo, Maiko Nakane for technical support on chiral amino acid analysis. We appreciate Steven D. Aird for editing the manuscript. Funding Information: This research was funded by Japan Agency for Medical Research and Development (AMED‐PRIME) (19gm6010017h0002) (JS), JSPS KAKENHI Grant number 21H02982 (JS), Shiseido. Co. Ltd. and Keio Gijuku Fukuzawa Memorial Fund for the Advancement of Education and Research (JS). Publisher Copyright: {\textcopyright} 2022 Federation of European Biochemical Societies.",
year = "2022",
month = nov,
doi = "10.1002/1873-3468.14417",
language = "English",
volume = "596",
pages = "2889--2897",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "22",
}