TY - JOUR
T1 - Asymmetric inheritance of radial glial fibers by cortical neurons
AU - Miyata, Takaki
AU - Kawaguchi, Ayano
AU - Okano, Hideyuki
AU - Ogawa, Masaharu
N1 - Funding Information:
We thank Drs. Richard S. Nowakowski, Katsuhiko Ono, and Toshio Ohshima for comments on an earlier version of the manuscript; and Drs. Verne S. Caviness, Jr., Takao Takahashi, Hiroyuki Ichijo, Tateharu Nagai, and Nobuaki Tamamaki for valuable discussion; Dr. Miyuki Yamamoto for RC2 antibody; and Dr. Masaki Inagaki for anti-phosphorylated vimentin. We also thank Masae Sato, Sachiko Yamamoto, Kanako Saito, Hideaki Miyashita, and Hiroshi Kuramochi for excellent technical assistance. This work was supported by grants from the Ministry of Education, Culture, Sports, Science, and Technology of the Japanese Government.
PY - 2001/9/13
Y1 - 2001/9/13
N2 - Recent studies demonstrated the neuronogenic role of radial glial cells (RGCs) in the rodent. To reveal the fate of radial glial processes, we intensively monitored divisions of RGCs in DiI-labeled slices from the embryonic day 14 mouse cortex. During RGC division, each pia-connected fiber becomes thin but is neither lost nor divided; it is inherited asymmetrically by one daughter cell. In divisions that produce a neuron and a progenitor, the neuron inherits the pial fiber, also grows a thick ventricular process for several hours, and is therefore indistinguishable from the progenitor RGC. The ventricular process in the radial glial-like neuron ("radial neuron") then collapses, leading to ascent of the neuron by using the "recycled" radial fiber.
AB - Recent studies demonstrated the neuronogenic role of radial glial cells (RGCs) in the rodent. To reveal the fate of radial glial processes, we intensively monitored divisions of RGCs in DiI-labeled slices from the embryonic day 14 mouse cortex. During RGC division, each pia-connected fiber becomes thin but is neither lost nor divided; it is inherited asymmetrically by one daughter cell. In divisions that produce a neuron and a progenitor, the neuron inherits the pial fiber, also grows a thick ventricular process for several hours, and is therefore indistinguishable from the progenitor RGC. The ventricular process in the radial glial-like neuron ("radial neuron") then collapses, leading to ascent of the neuron by using the "recycled" radial fiber.
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U2 - 10.1016/S0896-6273(01)00420-2
DO - 10.1016/S0896-6273(01)00420-2
M3 - Article
C2 - 11567613
AN - SCOPUS:0035855758
SN - 0896-6273
VL - 31
SP - 727
EP - 741
JO - Neuron
JF - Neuron
IS - 5
ER -