Autoantibodies to DNA-dependent protein kinase probes for the catalytic subunit

Akira Suwa, Michito Hirakata, Yoshihiko Takeda, Yutaka Okano, Tsuneyo Mimori, Shinichi Inada, Fumiaki Watanabe, Hirobumi Teraoka, William S. Dynan, John A. Hardin

研究成果: Article査読

31 被引用数 (Scopus)

抄録

DNA-dependent protein kinase (DNA-PK) is an important nuclear enzyme which consists of a catalytic subunit known as DNA-PKcs and a regulatory component identified as the Ku autoantigen. In the present study, we surveyed 312 patients in a search for this specificity. 10 sera immunoprecipitated a large polypeptide which exactly comigrated with DNA-PKcs in SDS-PAGE. Immunoblot analysis demonstrated that this polypeptide was recognizable by a rabbit antiserum specific for DNA-PKcs. Although the patient sera did not bind to biochemically purified DNA-PKcs in immunoblots or ELISA, they were able to deplete DNA-PK catalytic activity from extracts of HeLa cells in a dose-dependent manner. We conclude that these antibodies should be useful probes for studies which aim to define the role of DNA-PK in cells. Since six sera simultaneously contained antibodies to the Ku protein, these studies suggest that relatively intact forms of DNA-PK complex act as autoantigenic particles in selected patients.

本文言語English
ページ(範囲)1417-1421
ページ数5
ジャーナルJournal of Clinical Investigation
97
6
DOI
出版ステータスPublished - 1996 3月 15

ASJC Scopus subject areas

  • 医学一般

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