@article{cd3b300fda6d4f209ad072f47a40d0e5,
title = "Base editors for simultaneous introduction of C-to-T and A-to-G mutations",
abstract = "We describe base editors that combine both cytosine and adenine base-editing functions. A codon-optimized fusion of the cytosine deaminase PmCDA1, the adenosine deaminase TadA and a Cas9 nickase (Target-ACEmax) showed a high median simultaneous C-to-T and A-to-G editing activity at 47 genomic targets. On-target as well as DNA and RNA off-target activities of Target-ACEmax were similar to those of existing single-function base editors.",
author = "Sakata, {Rina C.} and Soh Ishiguro and Hideto Mori and Mamoru Tanaka and Kenji Tatsuno and Hiroki Ueda and Shogo Yamamoto and Motoaki Seki and Nanami Masuyama and Keiji Nishida and Hiroshi Nishimasu and Kazuharu Arakawa and Akihiko Kondo and Osamu Nureki and Masaru Tomita and Hiroyuki Aburatani and Nozomu Yachie",
note = "Funding Information: We thank members of the Yachie lab for useful discussions and critical assessment of this work, especially A. Adel for reviewing the manuscript. We also thank K. Shiina, Y. Takai and N. Ishii for technical supports of high-throughput sequencing. This study was mainly funded by the Uehara Memorial Foundation (to N.Y.), the NOVARTIS Foundation (Japan) for the Promotion of Science (to N.Y.), and the Japan Agency for Medical Research and Development (AMED) Platform Project for Supporting Drug Discovery and Life Science Research (to N.Y., H.N. and O.N.), and partly supported by the New Energy and Industrial Technology Development Organization (NEDO), AMED PRIME program (17gm6110007), the Japan Science and Technology Agency (JST) PRESTO program (10814), the Naito Foundation, the SECOM Science and Technology Foundation (all to N.Y.), the Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research (16J06287) (to S.I.) and research funds from the Yamagata Prefectural Government and Tsuruoka City, Japan (to K.A. and M. Tomita). S.I. was supported by a JSPS DC1 Fellowship; S.I., H.M. and N.M. were supported by TTCK Fellowships; H.M. and N.M. were supported by the Mori Memorial Foundation; and N.M. was supported by the Yamagishi Student Project Support Program of Keio University. Publisher Copyright: {\textcopyright} 2020, The Author(s), under exclusive licence to Springer Nature America, Inc.",
year = "2020",
month = jul,
day = "1",
doi = "10.1038/s41587-020-0509-0",
language = "English",
volume = "38",
pages = "865--869",
journal = "Nature Biotechnology",
issn = "1087-0156",
publisher = "Nature Publishing Group",
number = "7",
}