TY - JOUR
T1 - Bay K 8644, a voltage-sensitive calcium channel agonist, facilitates secretion of atrial natriuretic polypeptide from isolated perfused rat hearts
AU - Saito, Yoshihiko
AU - Nakao, Kazuwa
AU - Morri, Narito
AU - Sugawara, Akira
AU - Shiono, Shozo
AU - Yamada, Takayuki
AU - Itoh, Hiroshi
AU - Sakamito, Makoto
AU - Kurahashi, Kazuyoshi
AU - Fujiwara, Motohatsu
AU - Imura, Hiroo
N1 - Funding Information:
This work was supported in part by research grants from the Japanese Ministry of Education, Science and Culture, the Japanese Ministry of Health and Welfare "Disorders of Adrenal Hormone" Research Committee, Japan, 1985, Japan Tabacco Inc. and Yamanouchi Foundation for Research on Metabolic Disorders, by a research grant for cardiovascular diseases (60A-3) from the
Funding Information:
Japanese Ministry of Health and Welfare and by Japan Heart Foundation Research Grant For 1985.
PY - 1986/8/14
Y1 - 1986/8/14
N2 - Effects of Bay K 8644, a voltage-sensitive calcium channel agonist, on atrial natriuretic polypeptide (ANP) secretion from isolated rat hearts perfused with Krebs-Henseleit solution were investigated. After a ninetymin period for stabilization, coronary sinus effluents were collected every two min and ANP levels were measured by radioimmunoassay. The basal secretory rate of ANP was 1.65 ± 0.15 ng/min (mean ± standard error). Bay K 8644 stimulated ANP secretion dose-dependently. This stimulatory action was blocked by simultaneous administration of nifedipine, its competitive antagonist. Heart rate was also increased by Bay K 8644 administration. In the gel filtration study, the major secretory form of ANP corresponded to α-rat ANP, a 28-amino acid peptide. These results suggest that voltage-sensitive calcium channels are involved in two principal biological properties, contraction and ANP secretion, of atrial cardiocytes.
AB - Effects of Bay K 8644, a voltage-sensitive calcium channel agonist, on atrial natriuretic polypeptide (ANP) secretion from isolated rat hearts perfused with Krebs-Henseleit solution were investigated. After a ninetymin period for stabilization, coronary sinus effluents were collected every two min and ANP levels were measured by radioimmunoassay. The basal secretory rate of ANP was 1.65 ± 0.15 ng/min (mean ± standard error). Bay K 8644 stimulated ANP secretion dose-dependently. This stimulatory action was blocked by simultaneous administration of nifedipine, its competitive antagonist. Heart rate was also increased by Bay K 8644 administration. In the gel filtration study, the major secretory form of ANP corresponded to α-rat ANP, a 28-amino acid peptide. These results suggest that voltage-sensitive calcium channels are involved in two principal biological properties, contraction and ANP secretion, of atrial cardiocytes.
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U2 - 10.1016/S0006-291X(86)80405-3
DO - 10.1016/S0006-291X(86)80405-3
M3 - Article
C2 - 2428361
AN - SCOPUS:0022551581
SN - 0006-291X
VL - 138
SP - 1170
EP - 1176
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -