TY - JOUR
T1 - Better method for detection of CD30
T2 - Immunohistochemistry or flow cytometry?
AU - Higashi, Morihiro
AU - Kikuchi, Jun
AU - Murakami, Chiaki
AU - Takayanagi, Natsuko
AU - Momose, Shuji
AU - Kizaki, Masahiro
AU - Tamaru, Jun Ichi
N1 - Publisher Copyright:
© 2021 The Japanese Society for Lymphoreticular Tissue Research.
PY - 2021
Y1 - 2021
N2 - We compared the two methods of assessing CD30 protein expression in DLBCL and TCL specimens routinely employed at our hospital, immunohistochemistry (IHC) and flow cytometry (FCM), using the same clone of the anti-CD30 antibody (Ber-H2) in 123 patients with DLBCL and 28 patients with TCL. FCM was more sensitive than IHC, especially in cases with low expres-sion. In three cases of TCL and two cases of DLBCL, there was discordance between these two methods. Two of these TCL cases were ALCL and one was peripheral T-cell lymphoma, NOS, but ALCL was unable to be excluded. One of two cases of DLBCL was an anaplastic variant of DLBCL. The data suggested that CD30 was undetectable, though rare, by FMC in several cases. Based on this study, a combination of IHC and FCM is recommended for the reliable and quantitative detection of CD30.
AB - We compared the two methods of assessing CD30 protein expression in DLBCL and TCL specimens routinely employed at our hospital, immunohistochemistry (IHC) and flow cytometry (FCM), using the same clone of the anti-CD30 antibody (Ber-H2) in 123 patients with DLBCL and 28 patients with TCL. FCM was more sensitive than IHC, especially in cases with low expres-sion. In three cases of TCL and two cases of DLBCL, there was discordance between these two methods. Two of these TCL cases were ALCL and one was peripheral T-cell lymphoma, NOS, but ALCL was unable to be excluded. One of two cases of DLBCL was an anaplastic variant of DLBCL. The data suggested that CD30 was undetectable, though rare, by FMC in several cases. Based on this study, a combination of IHC and FCM is recommended for the reliable and quantitative detection of CD30.
KW - CD30
KW - T-cell lymphoma
KW - diffuse large B-cell lymphoma
KW - flow cytometry
KW - immunohistochemistry
UR - https://www.scopus.com/pages/publications/85147393409
UR - https://www.scopus.com/pages/publications/85147393409#tab=citedBy
U2 - 10.3960/jslrt.21019
DO - 10.3960/jslrt.21019
M3 - Article
AN - SCOPUS:85147393409
SN - 1346-4280
VL - 61
SP - 221
EP - 223
JO - Journal of Clinical and Experimental Hematopathology
JF - Journal of Clinical and Experimental Hematopathology
IS - 4
ER -