Brief report: Requirement of TACE/ADAM17 for hair follicle bulge niche establishment

Keisuke Nagao, Tetsuro Kobayashi, Manabu Ohyama, Haruhiko Akiyama, Keisuke Horiuchi, Masayuki Amagai

研究成果: Article査読

10 被引用数 (Scopus)

抄録

Hair follicles (HFs) are equipped with stem cell niches that allow regeneration. Tumor necrosis factor-α converting enzyme (TACE), also known as A disintegrin and metalloproteinase 17, is a proteolytic enzyme that regulates a variety of cell surface molecules including TNF-α, via ectodomain shedding. We found TACE expression on mouse HFs and conditionally depleted it in cells that expressed sex-determining region Y-related high-mobility-group box 9 (SOX9) transcription factor, an HF stem cell transcription factor (Taceflox/flox-Sox9-Cre, hereafter, "Tace/Sox9"). Tace/Sox9 mice were born with brittle hair with prolonged anagen phase. They underwent diffuse, progressive, and ultimately whole-body hair loss by 20 weeks old. Tace/Sox9 HFs lacked CD341 bulge cells as demonstrated via immunofluorescence microscopy and flow cytometry. Realtime PCR revealed downregulation of transcription factors Sox9, Lhx2, and Gata3 and upregulation of Lef1. In vitro colony-forming capacity was abolished in Tace/Sox9 keratinocytes, and HFs exhibited increased proliferation in situ, collectively demonstrating that Tace/Sox9 mice failed to establish the bulge niche and to maintain "stemness" of HF stem cells. Epidermal growth factor receptor (EGFR) signaling was impaired in Tace/Sox9 keratinocytes, and mice depleted of Egfr in SOX9-expressing tissues exhibited hair phenotype nearly identical to Tace/Sox9 mice, demonstrating EGFR signaling as a pathway downstream of TACE in HF homeostasis. This study provides mechanistic implication for human TACE-deficiency and for hair abnormality caused by EGFR inhibitors.

本文言語English
ページ(範囲)1781-1785
ページ数5
ジャーナルStem Cells
30
8
DOI
出版ステータスPublished - 2012 8月

ASJC Scopus subject areas

  • 分子医療
  • 発生生物学
  • 細胞生物学

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