Calibration and evaluation of routine methods by serum certified reference material for aldosterone measurement in blood

Tetsuo Nishikawa, Masao Omura, Migaku Kawaguchi, Akiko Takatsu, Fumitoshi Satoh, Sadayoshi Ito, Isao Kurihara, Hiroshi Itoh, Toshihiko Yanase, Hirotaka Shibata, Yutaka Oki, Mitsuhide Naruse, Keiko Sakurai, Hidehiko Sasamoto, Katsuhiko Kuwa

研究成果: Article査読

21 被引用数 (Scopus)


We attempted to study the standardization of aldosterone measurement in blood. The serum certified reference material (serum CRM) was established by spiking healthy human serum with pure aldosterone. ID-LC/MS/MS as a reference measurement procedure was performed by using the serum CRM. LC-MS/MS as a comparison method (CM) was routinely used for clinical samples, and the values with and without calibration by the serum CRM were compared. The serum CRM demonstrated similar reactivity with peripheral blood plasma as clinical samples in routine methods (RM) of RIA, ELISA, and CLEIA. In comparison between RM and CM, the results in regression analysis indicated that the range of the correlation coefficient (r) was 0.913-0.991, the range of y intercept was 0.9-67.3 pg/mL and the range of slope was 0.869-1.174. The values by RM in 100-150 pg/mL for the diagnostic level, had a significant calibration effect, and the relative difference between calibrated value in RM and result by CM was within ±20%. Furthermore, the calibrated value using the serum CRM was 10, 187 pg/mL, which corresponds to measured value of 14, 000 pg/mL using RIA for the adrenal venous sampling. Measured values between plasma and serum as a sample for the aldosterone measurement from clinical samples showed no significant differences. In conclusion, we succeeded to prepare the certified reference material of aldosterone for RM. Then, we can accurately calculate corrected values by using our equation for four RMs of determination of aldosterone.

ジャーナルEndocrine journal
出版ステータスPublished - 2016

ASJC Scopus subject areas

  • 内分泌学、糖尿病および代謝内科学
  • 内分泌学


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